Rapid development of nucleic acid diagnostics

There has been a significant increase, fueled by technologies front the human genome project, in the availability of nucleic acid sequence information for viruses and bacteria. This paper presents a computer-assisted process that begins with nucleic acid sequence information and produces highly spec...

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Bibliographic Details
Published in:Proceedings of the IEEE 2002-11, Vol.90 (11), p.1708-1721
Main Authors: Fitch, J.P., McCready, P.M., Gardner, S.N., Kuczmarski, T.A., Kurtz, S., Myers, R., Ott, L.L., Slezak, T.R., Vitalis, E.A., Zemla, A.T.
Format: Article
Language:English
Subjects:
Bioinformatics
Data engineering
Diseases
Foot-and-mouth disease virus
Genomics
Human immunodeficiency virus
Humans
Instruments
Microorganisms
Pathogens
Polymers
Viruses (medical)
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Summary:There has been a significant increase, fueled by technologies front the human genome project, in the availability of nucleic acid sequence information for viruses and bacteria. This paper presents a computer-assisted process that begins with nucleic acid sequence information and produces highly specific pathogen signatures. When combined with instrumentation using the polymerase chain reaction, the resulting diagnostics are both specific and sensitive. The computational and engineering aspects of converting raw sequence data into pathogen-specific and instrument-ready assays are presented. Examples and data are presented for specific pathogens, including foot-and-mouth disease virus and the human immunodeficiency virus.
ISSN:0018-9219
1558-2256
DOI:10.1109/JPROC.2002.804680