MyoBio: An Automated Bioreactor System Technology for Standardized Perfusion-Decellularization of Whole Skeletal Muscle

Objective: Decellularizing solid organs is a promising top-down process to produce acellular bio-scaffolds for 'de novo' regrowth or application as tissue 'patches' that compensate, e.g., large volumetric muscle loss in reconstructive surgery. Therefore, generating standardized a...

Full description

Saved in:
Bibliographic Details
Published in:IEEE transactions on biomedical engineering 2022-07, Vol.69 (7), p.2305-2313
Main Authors: Ritter, Paul, Cai, Aijia, Reischl, Barbara, Fiedler, Maren, Prols, Gerhard, Fries, Benjamin, Kretzschmar, Elke, Michael, Mena, Hartmann, Kristin, Lesko, Christian, Salti, Haitham, Arkudas, Andreas, Horch, Raymund, Paulsen, Friedrich, Friedrich, Oliver, Haug, Michael
Format: Article
Language:English
Subjects:
Arteries
Automation
Biological systems
Biomechatronics
Bioreactors
Biotechnology
decellularization
Deoxyribonucleic acid
DNA
Elasticity
Environmental assessment
Fluorescence
Inductors
Muscles
Organs
Perfusion
Plastic surgery
Plastics
Quality assessment
Reconstructive surgery
Regenerative medicine
Regrowth
Sarcomeres
Scaffolds
Second harmonic generation
Skeletal muscle
Surgery
Tendons
Tissue engineering
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Objective: Decellularizing solid organs is a promising top-down process to produce acellular bio-scaffolds for 'de novo' regrowth or application as tissue 'patches' that compensate, e.g., large volumetric muscle loss in reconstructive surgery. Therefore, generating standardized acellular muscle scaffolds marks a pressing area of need. Although animal muscle decellularization protocols were established, those are mostly manually performed and lack defined bioreactor environments and metrologies to assess decellularization quality in real-time. To close this gap, we engineered an automated bioreactor system to provide chemical decellularization solutions to immersed whole rat gastrocnemius medialis muscle through perfusion of the main feeding arteries. Results: Perfusion control is adjustable according to decellularization quality feedback. This was assessed both from (i) ex situ assessment of sarcomeres/nuclei through multiphoton fluorescence and label-free Second Harmonic Generation microscopy and DNA quantification, along with (ii) in situ within the bioreactor environment assessment of the sample's passive mechanical elasticity. Conclusion: We find DNA and sarcomere-free constructs after 72 h of 0.1% SDS perfusion-decellularization. Furthermore, passive elasticity can be implemented as additional online decellularization quality measure, noting a threefold elasticity decrease in acellular constructs. Significance: Our MyoBio represents a novel and useful automated bioreactor environment for standardized and controlled generation of acellular whole muscle scaffolds as a valuable source for regenerative medicine.
ISSN:0018-9294
1558-2531
DOI:10.1109/TBME.2022.3142317