Mycobacterium avium subspecies paratuberculosis and Crohn's disease granulomas

Objective. Chronic infection with Mycobacterium avium subspecies paratuberculosis (M. paratuberculosis) has been proposed as a cause of Crohn's disease. Although numerous investigators have examined the link between M. paratuberculosis and Crohn's disease, the evidence remains controversia...

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Bibliographic Details
Published in:Scandinavian journal of gastroenterology 2008, Vol.43 (9), p.1108-1111
Main Authors: Toracchio, Sonia, El-Zimaity, Hala M. T., Urmacher, Carlos, Katz, Seymour, Graham, David Y.
Format: Article
Language:English
Subjects:
Adult
Base Sequence
Biological and medical sciences
Crohn Disease - microbiology
Crohn Disease - pathology
Crohn's disease
DNA, Bacterial - analysis
Female
Gastroenterology. Liver. Pancreas. Abdomen
granuloma
Granuloma - microbiology
Granuloma - pathology
Humans
Intestinal Mucosa - microbiology
Intestinal Mucosa - pathology
IS900
Male
Medical sciences
Middle Aged
Molecular Sequence Data
Mycobacterium avium subsp. paratuberculosis - isolation & purification
Mycobacterium paratuberculosis
Other diseases. Semiology
Paraffin Embedding
paraffin-embedded
Paratuberculosis - diagnosis
Polymerase Chain Reaction
Probability
Reference Values
Retrospective Studies
Sensitivity and Specificity
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
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Summary:Objective. Chronic infection with Mycobacterium avium subspecies paratuberculosis (M. paratuberculosis) has been proposed as a cause of Crohn's disease. Although numerous investigators have examined the link between M. paratuberculosis and Crohn's disease, the evidence remains controversial. The aim of this study was to examine intestinal granuloma from Crohn's patients for M. paratuberculosis using a semi-nested M. paratuberculosis-specific IS900 polymerase chain reaction (PCR). Material and methods. Paraffin-embedded ileal or colonic tissues of patients with Crohn's disease were analyzed. Microdissection of this tissue into "granulomas" and "not granulomas" was performed. On the basis of sequences reported in GenBank alignments, we designed primer sets specific for M. paratuberculosis. The presence of the M. paratuberculosis was examined by semi-nested IS900-specific PCR with human -actin gene as a control for DNA quality. Results. Biopsies from 20 Crohn's patients were examined. Human -actin gene was amplified in all samples. M. paratuberculosis DNA was detected in the microdissected granuloma in 1 (5%) patient with Crohn's disease and in none of the "not granuloma" tissues. Conclusions. M. paratuberculosis DNA can rarely be detected within Crohn's granuloma. These results do not support M. paratuberculosis as the primary etiology of Crohn's disease.
ISSN:0036-5521
1502-7708
DOI:10.1080/00365520802116455