Effects of daunomycin implants on filtering surgery outcomes in rabbits

• Published in: Current eye research 1998-08, Vol.17 (8), p.844-850
• Main Authors: Morales, Jose, Kelleher, Peter J., Campbell, David, Crosson, Craig E.
• Format: Article
• Language: English
• Subjects: Animals; Anterior Eye Segment - drug effects; Antibiotics, Antineoplastic - pharmacokinetics; Antibiotics, Antineoplastic - pharmacology; daunomycin; Daunorubicin - pharmacokinetics; Daunorubicin - pharmacology; Drug Delivery Systems; Drug Implants; Filtering Surgery; glaucoma filtering surgery; Half-Life; hyaluronic acid; Hyaluronic Acid - pharmacology; intraocular pressure; Intraocular Pressure - drug effects; rabbit; Rabbits; Treatment Outcome
• ISSN: 0271-3683; 1460-2202
• DOI: 10.1080/02713689808951267

Purpose. To evaluate the effects of a novel daunomycin (DM) implant on intraocular pressure (TOP), bleb survival, and anterior segment complications when administered during filtering surgery on New Zealand White rabbits. Methods. Implants were prepared by covalent coupling of DM to a high molecular weight hyaluronic acid (HA) and fabricated into solid implants containing 250, 65, and 25 μg of DM. Full thickness sclerostomies were performed, and rabbits received no implant (control), placebo implant containing HA, or an implant containing HA-DM conjugate at the time of surgery. Rabbits were then followed for 30 days to assess change in IOP, bleb survival, and anterior segment associated complications. Results. In vitro, the release of DM from the implants was a first order process with a half-life of 51 h. In control rabbits, rabbits receiving placebo implant, and rabbits receiving HA-DM (250 μg) implants, the mean IOPs on day 3 were 11.1 ± 1.6, 10.8 ± 2.7, and 14 ± 0.98 mm of Hg, respectively. On days 5 through 9, IOP in the control and placebo-implanted groups returned to preoperative levels. However, in rabbits receiving 250 μg of conjugated DM, mean IOP on day 7 was reduced from preoperative levels by 11.8 ± 3.2 mm of Hg (P