An autoradiographic study of cochlea and organs of guinea pigs after intraperitoneal injection of 125I-labelled bFGF

• Published in: Acta oto-laryngologica 2010-03, Vol.130 (3), p.366-369
• Main Authors: Zhai, Suoqiang, Chen, Panzao, Guo, Weiwei, Yu, Ning, Sun, Jianhe, Hu, Yunyan
• Format: Article
• Language: English
• Subjects: Animals; Autoradiography; Biological and medical sciences; BLB; Blood-labyrinth barrier; Brain - diagnostic imaging; Brain - pathology; Capillary Permeability - physiology; Cochlea - diagnostic imaging; Cochlea - pathology; Fibroblast Growth Factor 2 - administration & dosage; Fibroblast Growth Factor 2 - metabolism; Guinea Pigs; Injections, Intraperitoneal; Iodine Radioisotopes - administration & dosage; Iodine Radioisotopes - metabolism; Kidney - diagnostic imaging; Kidney - pathology; Liver - diagnostic imaging; Liver - pathology; Medical sciences; Myocardium - pathology; Otorhinolaryngology. Stomatology; Radioimmunoassay; Radionuclide Imaging; Thyroid Gland - diagnostic imaging; Thyroid Gland - pathology; Tissue Distribution
• ISSN: 0001-6489; 1651-2251
• DOI: 10.3109/00016480903193437

Abstract Conclusion: The results of our research indicate that 125I-labelled basic fibroblast growth factor (125I-bFGF) has some difficulty in getting across the blood-labyrinth barrier (BLB). Therefore, it is necessary to carry out further study on the route of bFGF administration. Objective: To observe whether bFGF could pass through the BLB after intraperitoneal injection and to establish an experimental basis for its clinical applications. Methods: Thirty guinea pigs were divided into three equal groups. Animals in group 1 were administered 125I-bFGF, while animals in groups 2 and 3 were given 125I or saline solution, respectively, via intraperitoneal injection. The animals were sacrificed 2 h later and samples of blood, brain, cochlea, thyroid gland, heart, liver, and kidney were collected and weighed. A radioimmunoassay analyzer was employed to count the CPM values of each sample, and autoradiography was performed on section samples of both cochleae. Results: The CPM value of organ samples in the 125I group was higher than that in other groups, and radioactive grain was observed in cochlear samples of this group. In the 125I-bFGF group, blood demonstrated the highest CPM value, while cochlea and brain demonstrated the lowest CPM value, with no radioactive grain observed in cochlear samples.