A comparison of ejaculated and testicular spermatozoa aneuploidy rates in patients with high sperm DNA damage

Testicular spermatozoa are utilized to achieve pregnancy in couples with severe male factor infertility. Several studies suggest that aneuploidy rates in spermatozoa are elevated at the testicular level in infertile patients compared to ejaculates of normal controls. However, essential data regardin...

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Bibliographic Details
Published in:Systems biology in reproductive medicine 2012-06, Vol.58 (3), p.142-148
Main Authors: Moskovtsev, Sergey I., Alladin, Naazish, Lo, Kirk C., Jarvi, Keith, Mullen, J. Brendan M., Librach, Clifford L.
Format: Article
Language:English
Subjects:
Aneuploidy
Apoptosis
DNA Damage
Ejaculation - physiology
Humans
In Situ Hybridization, Fluorescence
Infertility, Male - etiology
Male
sperm DNA damage
Spermatozoa - pathology
Spermatozoa - physiology
testicular spermatozoa
Testis - pathology
TUNEL
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Summary:Testicular spermatozoa are utilized to achieve pregnancy in couples with severe male factor infertility. Several studies suggest that aneuploidy rates in spermatozoa are elevated at the testicular level in infertile patients compared to ejaculates of normal controls. However, essential data regarding aneuploidy rates between ejaculated and testicular spermatozoa in the same individuals is lacking. The purpose of our study was to compare aneuploidy rates at the testicular and post-testicular level from the same patients with persistently high sperm DNA damage. Ejaculates and testicular biopsies were obtained from eight patients with persistently high DNA damage (>30%). Both ejaculated and testicular samples were analyzed for sperm DNA damage and sperm aneuploidy for chromosomes 13, 18, 21, X, and Y. In addition, semen samples from ten normozoospermic men presenting for fertility evaluation served as a control group. A strong correlation between the alteration of spermatogenesis and chromatin deterioration was observed in our study. In the same individuals, testicular samples showed a significantly lower DNA damage compared to ejaculated spermatozoa (14.9% ± 5.0 vs. 40.6% ± 14.8, P 
ISSN:1939-6368
1939-6376
DOI:10.3109/19396368.2012.667504