Spectrofluorimetric Study of the Complexes Between Calcein and Lanthanide(III) Ions

The equilibrium of calcein, an H6L type fluorescent ligand, with lanthanide(III) ions, Ln(III), was studied spectrofluorimetrically in aqueous solution at constant ionic strength =0.1 (KC1), pH 8.0 and 25.0±0.1°C. Application of the mole ratio and continuous variation methods reveals the formation o...

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Bibliographic Details
Published in:Analytical letters 2000-01, Vol.33 (2), p.277-295
Main Authors: Benegi, Iñaki, del Campo, Gloria, Durand, J. Senén, Casado, J. Alfonso
Format: Article
Language:English
Subjects:
calcein
Chemistry
Exact sciences and technology
fluorexon
General and physical chemistry
lanthanides
rare earths
Solution properties
Solutions
spectrofluorimetry
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Summary:The equilibrium of calcein, an H6L type fluorescent ligand, with lanthanide(III) ions, Ln(III), was studied spectrofluorimetrically in aqueous solution at constant ionic strength =0.1 (KC1), pH 8.0 and 25.0±0.1°C. Application of the mole ratio and continuous variation methods reveals the formation of 1:1 complexes. The conditional stability constants (β') were calculated from the analysis of the observed fluorescence vs. [Ln(III)]/[calcein] mole ratio data by using an iterative non-linear least-squares computer program. The values obtained for β' are in the range 5.24×10 6 -5.77×10 7 The thermodynamic stability constant (β) were estimated by calculating the sidereaction coefficients (α) fro lanthanides and calcein. The β values obtained were from 3.2×10 12 to 3.6×10 13
ISSN:0003-2719
1532-236X
DOI:10.1080/00032710008543052