DEVELOPMENT OF LLE AND SPE PROCEDURES AND ITS APPLICATIONS FOR DETERMINATION OF OLMESARTAN IN HUMAN PLASMA USING RP-HPLC AND HPTLC

• Published in: Journal of liquid chromatography & related technologies 2010-01, Vol.33 (4), p.423-430
• Main Authors: Tambe, Santosh R., Shinde, Rupali H., Gupta, Lalit R., Pareek, Vikas, Bhalerao, Santosh B.
• Format: Article
• Language: English
• Subjects: Analytical chemistry; Chemistry; Chromatographic methods and physical methods associated with chromatography; Exact sciences and technology; HPTLC; human plasma; liquid-liquid extraction; Olmesartan Medoxomil; Other chromatographic methods; RP-HPLC; solid phase extraction
• ISSN: 1082-6076; 1520-572X
• DOI: 10.1080/10826070903571358

Two novel simple and rapid liquid-liquid and solid phase extraction methods have been developed for determination of olmesartan in human plasma using zidovudine as an internal standard. Liquid-liquid extraction from spiked human plasma samples was done using Dichloromethane: acetic acid (5.5: 0.5, v/v) solvent, while solid phase extraction was carried out on a DSC MCAX cartridge. For HPLC, the mobile phase consisted of water acetic acid pH 4.5 and methanol (25:75) at a flow rate of 0.5 mL · min −1 in isocratic mode. The calibration curve was plotted with a concentration range 10 μgmL −1 to 60 μgmL −1 . Recovery studies were carried out by LLE and SPE procedures with average recoveries 69.27% and 72.87%, respectively. For HPTLC the developing phase consisting of ethyl acetate methanol and acetic acid (8.0:2.0:0.05 v/v/v) and detection was carried out on 269 nm. The calibration curve was plotted over the concentration range of 200 ng to 600 ng. The average recoveries were 90.12% and 79.64% by LLE and SPE, respectively. The proposed method was validated as per US-FDA guidance.