Staining effect of Hibiscus sabdariffa extract on sperm cell morphology of Sprague-Dawley rats

There is increasing awareness among people towards natural products. Due to their non-toxic properties, low pollution, and lower side effects, natural dyes are used in many day-to-day products. Although the African continent possesses plentiful plant resources, only a small amount has been exploited...

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Bibliographic Details
Published in:Journal of histotechnology 2012-09, Vol.35 (3), p.110-113
Main Authors: Bassey, Rosemary B, Osinubi, Abraham A, Oremosu, Ademola A
Format: Article
Language:English
Subjects:
Dye
Hibiscus sabdariffa
Sperm cell
Stain
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Summary:There is increasing awareness among people towards natural products. Due to their non-toxic properties, low pollution, and lower side effects, natural dyes are used in many day-to-day products. Although the African continent possesses plentiful plant resources, only a small amount has been exploited so far. This study evaluated the use of Hibiscus sabdariffa as a stain to evaluate sperm morphology. Following liquefaction, 10 μl of semen was spread onto glass slides and allowed to air-dry at room temperature. The smear was fixed for 15 minutes in methanol. The sperm morphology was analyzed by staining 10 slides of the smears with eosin (control) and the ethanolic extract of Hibiscus sabdariffa dye was used to stain the sperm cells. The smears were air-dried and viewed at magnification of ×400. Phytochemical and chromatographic analyses were carried out. The sperm cells were stained in shades of reddish brown. Preliminary phytochemical screening of Hibiscus sabdariffa revealed that it contains alkaloids, saponins, flavonoids, and tannins. Hibiscus sabdariffa has potential for use as a stain for study of sperm morphology.
ISSN:0147-8885
2046-0236
DOI:10.1179/2046023612Y.0000000010