Classification and phylogenetic relationship of 10 fig varieties by RAPD and SSR molecular markers

• Published in: IOP conference series. Earth and environmental science 2019-10, Vol.332 (3), p.32026
• Main Authors: Zhang, Xiaoai, Hu, Yue, Liu, Liping, Wang, Xingteng, Wang, Weixin, Liu, Yuerong, Deng, Qunxian, Zhang, Huifen, Wang, Xun, Xia, Hui, Liang, Dong
• Format: Article
• Language: English
• Subjects: Cluster analysis; Correlation analysis; Genetic analysis; Genetic diversity; Loci; Markers; Phylogeny
• ISSN: 1755-1307; 1755-1315
• DOI: 10.1088/1755-1315/332/3/032026

In this experiment, 24 fig varieties were analyzed by RAPD and SSR. Results showed that 80 loci were amplified by 9 RAPD primers, including 70 polymorphic loci. The polymorphic percentage was 87.50%. A total of 34 allelic variations were detected in 10 pairs of SSR primers, 29 of which were polymorphic bands, and the percentage of polymorphic sites was 85.29%. Correlation analysis of the two markers of genetic identity showed significant correlation, indicating that the two markers were highly consistent in the analysis of fig genetic diversity. Cluster analysis revealed that the RAPD markers showed that the 24 fig varieties had a genetic similarity coefficient of 0.7 and could be divided into four groups. SSR markers showed that the 24 fig varieties can be divided into four groups at the genetic similarity coefficient of 0.66. The results show that the two molecular markers are suitable for the genetic diversity analysis of fig, and they are more reliable when combined than when used alone.