Radical reaction in aqueous media injected by atmospheric pressure plasma jet and protective effect of antioxidant reagents evaluated by single-molecule DNA measurement

Nonthermal atmospheric pressure plasma (NTP) has been extensively studied for biological and medical applications in recent years. Chemically active species are generated and injected into aqueous media subjected to plasma exposure. They play an important role in the activation and/or inactivation o...

Full description

Saved in:
Bibliographic Details
Published in:Japanese Journal of Applied Physics 2014, Vol.53 (5S1), p.05FR01-1-05FR01-4
Main Authors: Kurita, Hirofumi, Shimizu, Mika, Sano, Kaori, Nakajima, Tomoko, Yasuda, Hachiro, Takashima, Kazunori, Mizuno, Akira
Format: Article
Language:English
Subjects:
Antioxidants
Atmospheric pressure
Barometric pressure
Biomolecules
Deoxyribonucleic acid
Media
Plasma (physics)
Protective
Radicals
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Nonthermal atmospheric pressure plasma (NTP) has been extensively studied for biological and medical applications in recent years. Chemically active species are generated and injected into aqueous media subjected to plasma exposure. They play an important role in the activation and/or inactivation of biomolecules, such as nucleic acids, lipid, and proteins, in aqueous media. To evaluate the effect of NTP on these biomolecules at the molecular level, we have been considering large DNA molecules to be used as a biomarker. The single-molecule-based analysis of strand breakages induced by NTP, namely, the atmospheric pressure plasma jet (APPJ), on large DNA molecules has been performed. We demonstrated the facile estimation of the rate of double-strand breaks induced by the APPJ. Using this analysis, we have evaluated both the intensity of radical reaction and the protective effect on the reaction by adding antioxidant agents.
ISSN:0021-4922
1347-4065
DOI:10.7567/JJAP.53.05FR01