Morphometry and stereology of the conversion of thin-walled yeasts to phase I yeast cells of Wangiella dermatitidis

Morphometric and stereological analyses of the conversion of thin-walled yeast cells to thick-walled phase I yeast cells of Wangiella dermatitidis were conducted over 15 days of growth. Log phase growth persisted for 48-60 hours and there was no loss in viability up to 15 days. Average cell volume d...

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Bibliographic Details
Published in:Mycologia 1995-03, Vol.87 (2), p.153-160
Main Authors: Kester, A.S. (University of North Texas, Denton, TX.), Garrett, D.C
Format: Article
Language:English
Subjects:
Cell Biology/Ultrastructure
CELL DIFFERENTIATION
Cell growth
CELL STRUCTURE
CELL WALLS
CELLS
CELLULE
CELULAS
CRECIMIENTO
CROISSANCE
DEUTEROMYCOTINA
DEVELOPMENTAL STAGES
DIFERENCIACION CELULAR
DIFFERENCIATION CELLULAIRE
dimorphism
ESTRUCTURA CELULAR
ETAPAS DE DESARROLLO
Exophiala
Glycogen
GROWTH
GROWTH STAGES
Lipids
Material concentration
METHODE
METHODS
METODOS
MICROMORPHOMETRY
Mitochondria
MORFOGENESIS
MORPHOGENESE
MORPHOGENESIS
morphometry
Mother cells
PARED CELULAR
PAROI CELLULAIRE
polymorphism
STADE DE DEVELOPPEMENT
stereology
STRUCTURE CELLULAIRE
ULTRAESTRUCTURA
ULTRASTRUCTURE
Vacuoles
Wangiella
WANGIELLA DERMATITIDIS
Yeasts
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Summary:Morphometric and stereological analyses of the conversion of thin-walled yeast cells to thick-walled phase I yeast cells of Wangiella dermatitidis were conducted over 15 days of growth. Log phase growth persisted for 48-60 hours and there was no loss in viability up to 15 days. Average cell volume dropped from 23 μm 3 on days 1 and 2 to 18 μm 3 on days 3 and 4, then increased to 38 μm 3 by day 15. Wide confidence limits for measurements of cell volume at each point indicated a very heterogeneous population. Wall thickness increased throughout the growth period with thickness increasing from 0.04 μm to 0.30 μm and the volume fraction from 0.10 to 0.24. Mitochondria and nuclei together made up less than 8% of the cell volume [volume fraction of mitochondria, 0.025 (2.5%) and volume fraction of nuclei, 0.05 (5.0%)]. Volume fractions of both declined over time, as a result of increased cell volume from isotropic growth, although nuclei also may have become smaller. Glycogen was always present and increased to about 18% of the cell volume by day 4 but then decreased to 10% by day 15. Lipid was absent in early log phase cells but made up about 40% of the cell volume by day 15, and is presumably synthesized at the expense of glycogen and from residual sugar. Vacuoles, evident in early log cells (10-20% of the cell volume), declined rapidly and were rare after 8 days.
ISSN:0027-5514
1557-2536
DOI:10.1080/00275514.1995.12026516