Development of a Quasispecies of Human Immunodeficiency Virus Type 1 in vivo

During treatment with one specific batch of blood clotting factor IX, a number of hemophilia B patients in Germany recently became infected with human immunodeficiency virus type 1 (HIV-1). The nucleotide sequences of cloned HIV-1 envelope gene regions including the variable V3 loop and the V4 regio...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1992-08, Vol.89 (16), p.7365-7369
Main Authors: Cichutek, K., Merget, H., Norley, S., Linde, R., Kreuz, W., Gahr, M., Kurth, R.
Format: Article
Language:English
Subjects:
AIDS/HIV
Amino Acid Sequence
Base Sequence
Biological and medical sciences
Biological Evolution
Blood
Cloning, Molecular
DNA
DNA, Viral - genetics
DNA, Viral - isolation & purification
Drug Contamination
Factor IX - therapeutic use
Fundamental and applied biological sciences. Psychology
Genetic mutation
Genetic Variation
Genetics
Genotype
Genotypes
Geographic regions
Hemophilia A - therapy
HIV
HIV 1
HIV-1 - genetics
HIV-1 - isolation & purification
Human immunodeficiency virus
human immunodeficiency virus 1
Humans
Infections
Microbiology
Molecular Sequence Data
Nucleotides
Oligodeoxyribonucleotides
Polymerase Chain Reaction
Sequence Homology, Nucleic Acid
Virology
Viruses
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Summary:During treatment with one specific batch of blood clotting factor IX, a number of hemophilia B patients in Germany recently became infected with human immunodeficiency virus type 1 (HIV-1). The nucleotide sequences of cloned HIV-1 envelope gene regions including the variable V3 loop and the V4 region derived from short-term virus cultures and directly from peripheral blood cells of these patients were shown to be highly homologous. Based on the assumption that the corresponding consensus sequence (termed HIV-1MBK) was identical to the genotype of the initially infecting virus, we were able to construct phylogenetic trees of the developing quasispecies in two patients studied in detail. True intermediates between input and multiply mutated genotypes were found in individual blood samples. Except for the initially infecting variant HIV-1MBK, variants found at 11 months postinfection had replaced those seen at 5 months postinfection. Variability early after infection was shown to cluster in two small regions located 3' of the V3 loop (i.e., outside the loop) and within the V4 region. This communication therefore describes the evolution of an HIV-1 quasispecies in humans starting from a single genotype.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.89.16.7365