Functional Analysis of the ArabidopsisTETRASPANINGene Family in Plant Growth and Development

TETRASPANIN(TET) genes encode conserved integral membrane proteins that are known in animals to function in cellular communication during gamete fusion, immunity reaction, and pathogen recognition. In plants, functional information is limited to one of the 17 members of the Arabidopsis (Arabidopsis...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 2015-11, Vol.169 (3), p.2200-2214
Main Authors: Wang, Feng, Muto, Antonella, Van de Velde, Jan, Neyt, Pia, Himanen, Kristiina, Vandepoele, Klaas, Van Lijsebettens, Mieke
Format: Article
Language:English
Subjects:
Cell lines
Embryos
Genes
GENES, DEVELOPMENT, AND EVOLUTION
Guard cells
Phenotypes
Plant roots
Plants
Seedlings
Stem cells
Vascular tissues
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Summary:TETRASPANIN(TET) genes encode conserved integral membrane proteins that are known in animals to function in cellular communication during gamete fusion, immunity reaction, and pathogen recognition. In plants, functional information is limited to one of the 17 members of the Arabidopsis (Arabidopsis thaliana)TETgene family and to expression data in reproductive stages. Here, the promoter activity of all 17 ArabidopsisTETgenes was investigated bypAtTET::NUCLEAR LOCALIZATION SIGNAL-GREEN FLUORESCENT PROTEIN/β-GLUCURONIDASEreporter lines throughout the life cycle, which predicted functional divergence in the paralogous genes per clade. However, partial overlap was observed for manyTETgenes across the clades, correlating with few phenotypes in single mutants and, therefore, requiring double mutant combinations for functional investigation. Mutational analysis showed a role forTET13in primary root growth and lateral root development and redundant roles forTET5andTET6in leaf and root growth through negative regulation of cell proliferation. Strikingly, a number ofTETgenes were expressed in embryonic and seedling progenitor cells and remained expressed until the differentiation state in the mature plant, suggesting a dynamic function over developmental stages. The cis-regulatory elements together with transcription factor-binding data provided molecular insight into the sites, conditions, and perturbations that affectTETgene expression and positioned theTETgenes in different molecular pathways; the data represent a hypothesis-generating resource for further functional analyses.
ISSN:0032-0889
1532-2548