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Evidence That Ribosomal Protein S10 Itself is a Cellular Component Necessary for Transcription Antitermination by Phage λ N Protein
Bacteriophage λ N gene product acts to modify host RNA polymerase allowing the formation of a termination-resistant transcription apparatus. Previous studies have demonstrated that the nusE71 mutation that has altered the ribosomal protein S10 prevents N action in vivo. Using a coupled transcription...
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Published in: | Proceedings of the National Academy of Sciences - PNAS 1985-06, Vol.82 (12), p.4070-4074 |
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Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that cite this one |
Online Access: | Get full text |
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Summary: | Bacteriophage λ N gene product acts to modify host RNA polymerase allowing the formation of a termination-resistant transcription apparatus. Previous studies have demonstrated that the nusE71 mutation that has altered the ribosomal protein S10 prevents N action in vivo. Using a coupled transcription-translation system, we demonstrate here that purified S10 protein as well as the 30S ribosomal subunit is sufficient to restore N activity in the nusE mutant extract, allowing antitermination of Rho-dependent and Rho-independent terminators. This provides direct biochemical evidence that the S10 protein itself is one of the cellular components necessary for the formation of an antitermination apparatus. |
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ISSN: | 0027-8424 1091-6490 |
DOI: | 10.1073/pnas.82.12.4070 |