Host attachment and fluid shear are integrated into a mechanical signal regulating virulence inEscherichia coliO157:H7

EnterohemorrhagicEscherichia coli(EHEC) is a foodborne pathogen causing hemorrhagic colitis and hemolytic uremic syndrome. EHEC colonizes the intestinal tract through a range of virulence factors encoded by the locus of enterocyte effacement (LEE), as well as Shiga toxin. Although the factors involv...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2015-04, Vol.112 (17), p.5503-5508
Main Authors: Alsharif, Ghadah, Ahmad, Sadia, Islam, Md Shahidul, Shah, Riddhi, Busby, Stephen J., Krachler, Anne Marie
Format: Article
Language:English
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Summary:EnterohemorrhagicEscherichia coli(EHEC) is a foodborne pathogen causing hemorrhagic colitis and hemolytic uremic syndrome. EHEC colonizes the intestinal tract through a range of virulence factors encoded by the locus of enterocyte effacement (LEE), as well as Shiga toxin. Although the factors involved in colonization and disease are well characterized, how EHEC regulates its expression in response to a host encounter is not well understood. Here, we report that EHEC perceives attachment to host cells as a mechanical cue that leads to expression of LEE-encoded virulence genes. This signal is transduced via the LEE-encoded global regulator of LEE-encoded regulator (Ler) and global regulator of Ler and is further enhanced by levels of shear force similar to peristaltic forces in the intestinal tract. Our data suggest that, in addition to a range of chemical environmental signals, EHEC is capable of sensing and responding to mechanical cues to adapt to its host’s physiology.
ISSN:0027-8424
1091-6490