Improved herbivore resistance in cultivated tomato with the sesquiterpene biosynthetic pathway from a wild relative

Tomato breeding has been tremendously efficient in increasing fruit quality and quantity but did not focus on improving herbivore resistance. The biosynthetic pathway for the production of 7-epizingiberene in a wild tomato was introduced into a cultivated greenhouse variety with the aim to obtain he...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2012-12, Vol.109 (49), p.20124-20129
Main Authors: Bleeker, Petra M, Mirabella, Rossana, Diergaarde, Paul J, VanDoorn, Arjen, Tissier, Alain, Kant, Merijn R, Prins, Marcel, de Vos, Martin, Haring, Michel A, Schuurink, Robert C
Format: Article
Language:English
Subjects:
Alkyl and Aryl Transferases - genetics
Alkyl and Aryl Transferases - metabolism
Analysis of Variance
Animals
biochemical pathways
Biological Sciences
Biosynthesis
Biosynthetic Pathways - genetics
breeding
Breeding - methods
Cloning, Molecular
Eggs
Feeding Behavior - physiology
fruit quality
Gas Chromatography-Mass Spectrometry
greenhouses
Hemiptera - physiology
Herbivores
Herbivory - immunology
Lycopersicon esculentum - genetics
Lycopersicon esculentum - immunology
Lycopersicon esculentum - parasitology
Metabolic Engineering - methods
Mites
Monoterpenes
pests
Plants
Plastids
Polyisoprenyl Phosphates
Real-Time Polymerase Chain Reaction
Sesquiterpenes
Sesquiterpenes - immunology
Sesquiterpenes - metabolism
sesquiterpenoids
Spiders
Terpenes
Tetranychidae - physiology
Tomatoes
toxicity
Transgenic plants
Trichomes
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Summary:Tomato breeding has been tremendously efficient in increasing fruit quality and quantity but did not focus on improving herbivore resistance. The biosynthetic pathway for the production of 7-epizingiberene in a wild tomato was introduced into a cultivated greenhouse variety with the aim to obtain herbivore resistance. 7-Epizingiberene is a specific sesquiterpene with toxic and repellent properties that is produced and stored in glandular trichomes. We identified 7-epizingiberene synthase (ShZIS) that belongs to a new class of sesquiterpene synthases, exclusively using Z-Z- farnesyl-diphosphate (zFPP) in plastids, probably arisen through neo-functionalization of a common ancestor. Expression of the ShZIS and zFPP synthases in the glandular trichomes of cultivated tomato resulted in the production of 7-epizingiberene. These tomatoes gained resistance to several herbivores that are pests of tomato. Hence, introduction of this sesquiterpene biosynthetic pathway into cultivated tomatoes resulted in improved herbivore resistance.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.1208756109