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Synthesis of DNA in Human Lymphocytes: Possible Control Mechanism
Partially purified, isolated nuclei from lymphocytes either stimulated or not stimulated by phytohemagglutinin can equally well synthesize DNA when [3H]dTTP is used as precursor. Studies of DNA polymerase activity in nuclei and cytoplasm from these cells showed that the enzyme can be detected in eit...
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| Published in: | Proceedings of the National Academy of Sciences - PNAS 1974-04, Vol.71 (4), p.1128-1132 |
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| Main Authors: | , , |
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| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c456t-c0be6d642af47b5628b7b42918612b7345c1e2f3c54384efa8d595342726e3143 |
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| container_end_page | 1132 |
| container_issue | 4 |
| container_start_page | 1128 |
| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 71 |
| creator | Fridlender, Bertold R. Medrano, Estela Mordoh, Jose |
| description | Partially purified, isolated nuclei from lymphocytes either stimulated or not stimulated by phytohemagglutinin can equally well synthesize DNA when [3H]dTTP is used as precursor. Studies of DNA polymerase activity in nuclei and cytoplasm from these cells showed that the enzyme can be detected in either stimulated or nonstimulated lymphocytes. In nonstimulated lymphocytes the uptake of thymidine is very low. The use of inhibitors such as cycloheximide, arabinosylcytosine, and actinomycin D showed that a parallel existed between thymidine uptake and DNA synthesis. All the conditions in which DNA synthesis was inhibited resulted also in an inhibition of thymidine uptake. |
| doi_str_mv | 10.1073/pnas.71.4.1128 |
| format | article |
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Studies of DNA polymerase activity in nuclei and cytoplasm from these cells showed that the enzyme can be detected in either stimulated or nonstimulated lymphocytes. In nonstimulated lymphocytes the uptake of thymidine is very low. The use of inhibitors such as cycloheximide, arabinosylcytosine, and actinomycin D showed that a parallel existed between thymidine uptake and DNA synthesis. All the conditions in which DNA synthesis was inhibited resulted also in an inhibition of thymidine uptake.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.71.4.1128</identifier><identifier>PMID: 4524623</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Actinomycin ; Biological Sciences: Cell Biology ; Cell Fractionation ; Cell nucleus ; Cell Nucleus - drug effects ; Cell Nucleus - metabolism ; Cells ; Chemical suspensions ; Cycloheximide - pharmacology ; Cytarabine - pharmacology ; Cytoplasm - drug effects ; Cytoplasm - metabolism ; Dactinomycin - pharmacology ; DNA ; DNA - biosynthesis ; DNA Nucleotidyltransferases - metabolism ; DNA Replication - drug effects ; Enzymes ; Homogenization ; Humans ; Lectins - pharmacology ; Lymphocytes ; Lymphocytes - cytology ; Lymphocytes - drug effects ; Lymphocytes - enzymology ; Lymphocytes - metabolism ; Nucleic acid precursors ; Stem cells ; Stimulation, Chemical ; Thymidine - metabolism ; Thymine Nucleotides - metabolism ; Tritium</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1974-04, Vol.71 (4), p.1128-1132</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c456t-c0be6d642af47b5628b7b42918612b7345c1e2f3c54384efa8d595342726e3143</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/71/4.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/63276$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/63276$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793,58238,58471</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4524623$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fridlender, Bertold R.</creatorcontrib><creatorcontrib>Medrano, Estela</creatorcontrib><creatorcontrib>Mordoh, Jose</creatorcontrib><title>Synthesis of DNA in Human Lymphocytes: Possible Control Mechanism</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Partially purified, isolated nuclei from lymphocytes either stimulated or not stimulated by phytohemagglutinin can equally well synthesize DNA when [3H]dTTP is used as precursor. Studies of DNA polymerase activity in nuclei and cytoplasm from these cells showed that the enzyme can be detected in either stimulated or nonstimulated lymphocytes. In nonstimulated lymphocytes the uptake of thymidine is very low. The use of inhibitors such as cycloheximide, arabinosylcytosine, and actinomycin D showed that a parallel existed between thymidine uptake and DNA synthesis. All the conditions in which DNA synthesis was inhibited resulted also in an inhibition of thymidine uptake.</description><subject>Actinomycin</subject><subject>Biological Sciences: Cell Biology</subject><subject>Cell Fractionation</subject><subject>Cell nucleus</subject><subject>Cell Nucleus - drug effects</subject><subject>Cell Nucleus - metabolism</subject><subject>Cells</subject><subject>Chemical suspensions</subject><subject>Cycloheximide - pharmacology</subject><subject>Cytarabine - pharmacology</subject><subject>Cytoplasm - drug effects</subject><subject>Cytoplasm - metabolism</subject><subject>Dactinomycin - pharmacology</subject><subject>DNA</subject><subject>DNA - biosynthesis</subject><subject>DNA Nucleotidyltransferases - metabolism</subject><subject>DNA Replication - drug effects</subject><subject>Enzymes</subject><subject>Homogenization</subject><subject>Humans</subject><subject>Lectins - pharmacology</subject><subject>Lymphocytes</subject><subject>Lymphocytes - cytology</subject><subject>Lymphocytes - drug effects</subject><subject>Lymphocytes - enzymology</subject><subject>Lymphocytes - metabolism</subject><subject>Nucleic acid precursors</subject><subject>Stem cells</subject><subject>Stimulation, Chemical</subject><subject>Thymidine - metabolism</subject><subject>Thymine Nucleotides - metabolism</subject><subject>Tritium</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1974</creationdate><recordtype>article</recordtype><recordid>eNp9kDtPwzAURi0EKqWwMiAhZeqW4MeN7SAxVOVRpPKQgNlyXIcGJXGJE0T_PalaqrIwefjOd33vQeiU4IhgwS4WlfaRIBFEhFC5h_oEJyTkkOB91MeYilAChUN05P0HxjiJJe6hHsQUOGV9NHpZVs3c-twHLguuH0dBXgWTttRVMF2Wi7kzy8b6y-DZeZ-nhQ3GrmpqVwQP1sx1lfvyGB1kuvD2ZPMO0Nvtzet4Ek6f7u7Ho2loIOZNaHBq-YwD1RmINOZUpiIFmhDJCU0Fg9gQSzNmYmASbKblLE5iBlRQbhkBNkBX67mLNi3tzNhuD12oRZ2Xul4qp3P1N6nyuXp3X4pJSQTv-sNNv3afrfWNKnNvbFHoyrrWK0kBi85XB0Zr0NTd0bXNtn8QrFbO1cq5EkSBWjnvCue7m23xjeSdfNX7TXf7w_9ylbVF0djvpgPP1uCHb1y9JTmj3X0_OnidTg</recordid><startdate>19740401</startdate><enddate>19740401</enddate><creator>Fridlender, Bertold R.</creator><creator>Medrano, Estela</creator><creator>Mordoh, Jose</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19740401</creationdate><title>Synthesis of DNA in Human Lymphocytes: Possible Control Mechanism</title><author>Fridlender, Bertold R. ; Medrano, Estela ; Mordoh, Jose</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c456t-c0be6d642af47b5628b7b42918612b7345c1e2f3c54384efa8d595342726e3143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1974</creationdate><topic>Actinomycin</topic><topic>Biological Sciences: Cell Biology</topic><topic>Cell Fractionation</topic><topic>Cell nucleus</topic><topic>Cell Nucleus - drug effects</topic><topic>Cell Nucleus - metabolism</topic><topic>Cells</topic><topic>Chemical suspensions</topic><topic>Cycloheximide - pharmacology</topic><topic>Cytarabine - pharmacology</topic><topic>Cytoplasm - drug effects</topic><topic>Cytoplasm - metabolism</topic><topic>Dactinomycin - pharmacology</topic><topic>DNA</topic><topic>DNA - biosynthesis</topic><topic>DNA Nucleotidyltransferases - metabolism</topic><topic>DNA Replication - drug effects</topic><topic>Enzymes</topic><topic>Homogenization</topic><topic>Humans</topic><topic>Lectins - pharmacology</topic><topic>Lymphocytes</topic><topic>Lymphocytes - cytology</topic><topic>Lymphocytes - drug effects</topic><topic>Lymphocytes - enzymology</topic><topic>Lymphocytes - metabolism</topic><topic>Nucleic acid precursors</topic><topic>Stem cells</topic><topic>Stimulation, Chemical</topic><topic>Thymidine - metabolism</topic><topic>Thymine Nucleotides - metabolism</topic><topic>Tritium</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fridlender, Bertold R.</creatorcontrib><creatorcontrib>Medrano, Estela</creatorcontrib><creatorcontrib>Mordoh, Jose</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fridlender, Bertold R.</au><au>Medrano, Estela</au><au>Mordoh, Jose</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synthesis of DNA in Human Lymphocytes: Possible Control Mechanism</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1974-04-01</date><risdate>1974</risdate><volume>71</volume><issue>4</issue><spage>1128</spage><epage>1132</epage><pages>1128-1132</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Partially purified, isolated nuclei from lymphocytes either stimulated or not stimulated by phytohemagglutinin can equally well synthesize DNA when [3H]dTTP is used as precursor. Studies of DNA polymerase activity in nuclei and cytoplasm from these cells showed that the enzyme can be detected in either stimulated or nonstimulated lymphocytes. In nonstimulated lymphocytes the uptake of thymidine is very low. The use of inhibitors such as cycloheximide, arabinosylcytosine, and actinomycin D showed that a parallel existed between thymidine uptake and DNA synthesis. All the conditions in which DNA synthesis was inhibited resulted also in an inhibition of thymidine uptake.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>4524623</pmid><doi>10.1073/pnas.71.4.1128</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1974-04, Vol.71 (4), p.1128-1132 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_jstor_primary_63276 |
| source | JSTOR Archival Journals and Primary Sources Collection; PubMed Central |
| subjects | Actinomycin Biological Sciences: Cell Biology Cell Fractionation Cell nucleus Cell Nucleus - drug effects Cell Nucleus - metabolism Cells Chemical suspensions Cycloheximide - pharmacology Cytarabine - pharmacology Cytoplasm - drug effects Cytoplasm - metabolism Dactinomycin - pharmacology DNA DNA - biosynthesis DNA Nucleotidyltransferases - metabolism DNA Replication - drug effects Enzymes Homogenization Humans Lectins - pharmacology Lymphocytes Lymphocytes - cytology Lymphocytes - drug effects Lymphocytes - enzymology Lymphocytes - metabolism Nucleic acid precursors Stem cells Stimulation, Chemical Thymidine - metabolism Thymine Nucleotides - metabolism Tritium |
| title | Synthesis of DNA in Human Lymphocytes: Possible Control Mechanism |
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