Application of a Time–Resolved Fluoroimmunoassay for the Analysis of Normal Prion Protein in Human Blood and Its Components

Background and Objectives: To quantify the cellular isoform of prion protein (PrP c ) in human blood using a new time–resolved dissociation–enhanced fluoroimmunoassay (DELFIA ® ). Materials and Methods: The DELFIA was optimised for human blood samples and applied to isolated cell and plasma fraction...

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Bibliographic Details
Published in:Vox sanguinis 1999-09, Vol.77 (2), p.88-96
Main Authors: MacGregor, Ian, Hope, James, Barnard, Geoff, Kirby, Louise, Drummond, Olive, Pepper, Duncan, Hornsey, Valerie, Barclay, Robin, Bessos, Hagop, Turner, Marc, Prowse, Christopher
Format: Article
Language:English
Subjects:
Transfusion, Microbiology and Plasma Fractions
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Summary:Background and Objectives: To quantify the cellular isoform of prion protein (PrP c ) in human blood using a new time–resolved dissociation–enhanced fluoroimmunoassay (DELFIA ® ). Materials and Methods: The DELFIA was optimised for human blood samples and applied to isolated cell and plasma fractions from blood donations. The physicochemical properties of PrP c were analysed. Results: 26.5% of blood PrP c was associated with the platelet fraction, 0.8% with polymorphonuclear leucocytes, 2.4% with mononuclear leucocytes, 1.8% with red cells and 68.5% with plasma (mean values from 4 processed donations). Conclusion: The majority of blood PrP c is found in the platelet and plasma compartments.
ISSN:0042-9007
1423-0410
DOI:10.1159/000031082