Pharmacological approaches for calcium mobilization by vitisin C, a novel plant oligostilbene from Vitis coignetiae in the endothelial cells from rat aorta

• Published in: Japanese Journal of Pharmacology 2001, Vol.85 (suppl.1), p.70-70
• Main Authors: Kazuhiko Seya, Ken-ichi Furukawa, Satoshi Taniguchi, Yoshiaki Takaya, Yoshiteru Oshima, Masatake Niwa, Shigeru Motomura
• Format: Article
• Language: Japanese
• ISSN: 0021-5198

In the previous studies, we revealed that vitisin C, a novel plant oligostilbene from Vitis coignetiae inhibited the contractile responses of rabbit aorta in an endothelium-dependent manner by producing nitric oxide. To confirm the calcium mobilization of vitisin C, we investigated calcium influx by vitisin C into the endothelial cells prepared from rabbit aorta and compared its effect with carbachol and A23187. The effective concentrations of these compounds producing approximately 50% of the maximal relaxation response on the phenylephrine (1μM) -precontricted endothelial-intact rabbit aorta were used for investigation. All of vitisin C (3μM), carbachol (1μM), and A23187 (3 nM) increased ^^45 Ca influx into the endothelial cells. In the presence of SK & F 96365, a potent blocker of receptor-operated calcium channels, the ^^45 Ca influx induced by carbachol and vitisin C but not A23187 was inhibited. However, nifedipine, a blocker of voltage dependent calcium channels, inhibited ^^45 Ca influx induced by vitisin C and A23187. These results suggest that vitisin C induces nitric oxide production via activation of calcium influx into the endothelial cells, resulting in the endothelial-dependent inhibition of the contractile responses of rabbit aorta.