O-20118 Effects of DIDS and chlorpromazine on NO3- transport in erythrocytes：Inertness of DIDS in whole blood

Higher NOx concentrations in washed erythrocytes (RBC) of patients with schizophrenia than that of control subjects has been indicated. However, this was inconsistent with our experience that washing procedure could partially or completely reduce RBC NOx to a level equivalent to that in the washing...

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Published in:Journal of Pharmacological Sciences 2004, Vol.94 (suppl.1), p.103-103
Main Authors: Takaharu Ishibashi, Kaname Kubota, Junko Yoshida, Matomo Nishio
Format: Article
Language:Japanese
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Summary:Higher NOx concentrations in washed erythrocytes (RBC) of patients with schizophrenia than that of control subjects has been indicated. However, this was inconsistent with our experience that washing procedure could partially or completely reduce RBC NOx to a level equivalent to that in the washing solution. Therefore, we examined the effects of chlorpromazine (one of major agents used in the schizophrenic patients) on NO3- transport to elucidate whether or not this agent retains NO3- within RBC. Chlorpromazine (10 μg/ml) did not influence NO3- movement in both whole blood and RBC suspension. Though an anion exchanger (AE1) inhibitor. DIDS (4, 4'-diisothiocyanato-stilbene-2, 2'-disulfonic acid, 100 μM), did not alter NO3- movement in whole blood, it inhibited the movement in RBC suspension in a concentration-dependent manner. The inhibition was abrogated by plasma and albumin concentration-dependently. These results indicated that chlorpromazine had no effect on NO3- transport through AE1, and that inertness of DIDS on AE1 in whole blood was due to interference by albumin in plasma.
ISSN:1347-8613