Overexpression of TRIM44 mediates the NF-κB pathway to promote the progression of ovarian cancer

• Published in: Genes & genomics 2024, 46(6), , pp.689-699
• Main Authors: Yu, Yang, Li, ShiYing, Sun, Jialin, Wang, Yu, Xie, LiangZhen, Guo, Ying, Li, Jia, Han, FengJuan
• Format: Article
• Language: English
• Subjects: Animal Genetics and Genomics; Animals; Apoptosis; Apoptosis - genetics; Biomedical and Life Sciences; Cell Line, Tumor; Cell migration; Cell Movement - genetics; Cell proliferation; Cell Proliferation - genetics; Cholecystokinin; Disease Progression; Enzyme-linked immunosorbent assay; Female; Gene Expression Regulation, Neoplastic; Human Genetics; Humans; Immunofluorescence; Intracellular Signaling Peptides and Proteins - genetics; Intracellular Signaling Peptides and Proteins - metabolism; Life Sciences; Mice; Mice, Inbred BALB C; Mice, Nude; Microbial Genetics and Genomics; Molecular modelling; NF-kappa B - genetics; NF-kappa B - metabolism; NF-κB protein; Ovarian cancer; Ovarian Neoplasms - genetics; Ovarian Neoplasms - metabolism; Ovarian Neoplasms - pathology; Plant Genetics and Genomics; Research Article; Signal transduction; Signal Transduction - genetics; Therapeutic targets; Tripartite Motif Proteins - genetics; Tripartite Motif Proteins - metabolism; Tumors; 생물학
• ISSN: 1976-9571; 2092-9293; 2092-9293
• DOI: 10.1007/s13258-024-01517-7

Background Ovarian cancer (OC) is the second most commonly seen cancer in the US, and patients with OC are commonly diagnosed in the advanced stage. Research into the molecular mechanisms and potential therapeutic targets of OC is becoming increasingly urgent. In our study, we worked to discover the role of TRIM44 in OC development. Objective This study explored whether the overexpression of TRIM44 mediates the NF-kB pathway to promote the progression of OC. Methods A TRIM44 overexpression model was constructed in SKOV3 cells, and the proliferation ability of the cells was detected using the CCK-8 assay. The migration healing ability of cells was detected using cell scratch assay. Cell migration and invasion were detected using Transwell nesting. TUNEL was applied to detect apoptosis, and ELISA and western blot were used to detect the expression of NF-κB signaling pathway proteins. The pathological changes of the tumor tissues were observed using HE staining in a mouse ovarian cancer xenograft model. Immunofluorescence double staining, RT-PCR, and western blot were used to determine the expression of relevant factors in tumour tissues. Results TRIM44 overexpression promoted the proliferation, migration, and invasion of SKOV3 cells in vitro and inhibited apoptosis while enhancing the growth of tumours in vivo. TRIM44 regulated the NF-κB signaling pathway. Conclusions TRIM44 overexpression can regulate the NF-κB signaling pathway to promote the progression of OC, and TRIM44 may be a potential therapeutic target for OC.