Epigenetic modification of retinoic acid-treated human embryonic stem cells

Epigenetic modification of the genome through DNA methylation is the key to maintaining the differentiated state of human embryonic stem cells (hESCs), and it must be reset during differentiation by retinoic acid (RA) treatment. A genome-wide methylation/gene expression assay was performed in order...

Full description

Saved in:
Bibliographic Details
Published in:BMB reports 2010, 43(12), , pp.830-835
Main Authors: Cheong, H.S., SNP Genetics, Inc., Seoul, Republic of Korea, Lee, H.C., Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea, Park, B.L., SNP Genetics, Inc., Seoul, Republic of Korea, Kim, H.M., Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea, Jang, M.J., Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea, Han, Y.M., Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea, Kim, S.Y., Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea, Kim, Y.S., Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea, Shin, H.D., SNP Genetics, Inc., Seoul, Republic of Korea
Format: Article
Language:English
Subjects:
DNA methylation
Epigenetic modification
EXPRESION GENICA
EXPRESSION DES GENES
GENE EXPRESSION
Human embryonic stem cell
Retinoic acid
화학
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Epigenetic modification of the genome through DNA methylation is the key to maintaining the differentiated state of human embryonic stem cells (hESCs), and it must be reset during differentiation by retinoic acid (RA) treatment. A genome-wide methylation/gene expression assay was performed in order to identify epigenetic modifications of RA-treated hESCs. Between undifferentiated and RA-treated hESCs, 166 differentially methylated CpG sites and 2,013 differentially expressed genes were discovered. Combined analysis of methylation and expression data revealed that 19 genes (STAP2, VAMP8, C10orf26, WFIKKN1, ELF3, C1QTNF6, C10orf10, MRGPRF, ARSE, LSAMP, CENTD3, LDB2, POU5F1, GSPT2, THY1, ZNF574, MSX1, SCMH1, and RARB) were highly correlated with each other. The results provided in this study will facilitate future investigations into the interplay between DNA methylation and gene expression through further functional and biological studies.
ISSN:1976-6696
1976-670X
DOI:10.5483/BMBRep.2010.43.12.830