Determination of eperisone in human plasma by liquid chromatography-ESI-tandem mass spectrometry

A sensitive and selective method for the determination of 4'-ethyl-3-methyl-3-piperidinopropiophenone hydrochloride (eperisone hydrochloride) in human plasma was developed and validated. The procedure employed an internal standard and a solvent extraction step followed by chromatography on a Xt...

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Bibliographic Details
Published in:Archives of pharmacal research 2007, 30(9), , pp.1174-1178
Main Authors: Jeoung, Min Kyo, Jeong, Eun Sook, Kim, Nam Hee, Kim, Chang-Soo, Chung, Youn-Bok, Lee, Yong-Moon, Ahn, Su-Youn, Cho, Hwang-Eui, Lee, Yong Hwa, Hong, Jin Tae, Moon, Dong-Cheul
Format: Article
Language:English
Subjects:
Adult
Area Under Curve
Calibration
Chromatography, High Pressure Liquid - methods
Humans
Male
Propiophenones - blood
Spectrometry, Mass, Electrospray Ionization - methods
약학
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Summary:A sensitive and selective method for the determination of 4'-ethyl-3-methyl-3-piperidinopropiophenone hydrochloride (eperisone hydrochloride) in human plasma was developed and validated. The procedure employed an internal standard and a solvent extraction step followed by chromatography on a Xterra C18 minibore column. Detection was by electrospray ionization tandem mass spectrometry with multiple reaction monitoring. The mass transitions of eperisone and tolperisone (IS) were m/z 260 --> 98 and m/z 246 --> 98, respectively. The method has a limit of detection of 0.1 pg/mL for eperisone based on the three times signal-to-noise value with a linear range from 0.01 to 10.0 ng/mL for the analyte. Extraction recovery was on average 98.6 +/- 7.2% (SD) for eperisone. The Intra- and inter-day assay accuracy ranged from 93 to 114% and precision (RSD) was better than 8.5%. The method was successfully employed to analyze plasma samples and evaluate pharmacokinetics of eperisone in healthy male volunteers.
ISSN:0253-6269
1976-3786
DOI:10.1007/bf02980255