Controllable mouse epidermal growth factor (mEGF) release by photo-encapsulation using azidophenyl chitosan derivative and its wound healing effect

Photoreactive azidophenyl chitosan was synthesized and used to encapsulate mouse epidermal growth factor (mEGF). Cytotoxicity assays were carried out and the release of the encapsulated mEGF was observed. As increasing the number of azidophenyl chitosan layers, relatively longer and gentler release...

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Bibliographic Details
Published in:Macromolecular research 2016, 24(10), , pp.862-867
Main Authors: Kim, Kwang-Il, Han, Ga-Dug, Kim, Eun-Hye, Jeong, Jin-Hong, Lee, Jae-Woo, Kim, Mi-Kyung, Ito, Yoshihiro, Son, Tae-Il
Format: Article
Language:English
Subjects:
Biocompatibility
Cell growth
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Chitosan
Complex Fluids and Microfluidics
Controllability
Encapsulation
Epidermal growth factor
Growth factors
In vivo methods and tests
Nanochemistry
Nanotechnology
Physical Chemistry
Polymer Sciences
Proteinase
Soft and Granular Matter
Ultraviolet radiation
Wound healing
고분자공학
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Summary:Photoreactive azidophenyl chitosan was synthesized and used to encapsulate mouse epidermal growth factor (mEGF). Cytotoxicity assays were carried out and the release of the encapsulated mEGF was observed. As increasing the number of azidophenyl chitosan layers, relatively longer and gentler release was observed. The activity of encapsulated mEGF was examined by measuring 3T3 cell proliferation in the presence or absence of proteinase-K. The in vivo activity of encapsulated mEGF was also examined in rats. The UV light used for these experiments did not affect on the cell growth-enhancing property of mEGF, and encapsulated mEGF was more stable than free mEGF. In the wounds treated with photo-encapsulated mEGF showed the lower inflammation and the better regenerative effect than others. From the in vivo test, it is considered that photo-encapsulation of mEGF by azidophenyl chitosan could accelerate the initial stage of wound healing. Also, it would be expected to apply to current medical devices to upgrade.
ISSN:1598-5032
2092-7673
DOI:10.1007/s13233-016-4125-2