Antioxidant and cognitive-enhancing activities of Arctium lappa L. roots in Aβ1-42-induced mouse model

Many studies have shown that oxidative stress induced by hydrogen peroxide can lead to neuronal cell death. In this study, protective effects of the ethanol extract of A. lappa L. roots against oxidative stress in PC12 cells were measured by 2′, 7′-dichlorofluorescein diacetate and 3-(4,5-dimethylth...

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Published in:Applied biological chemistry 2016, 59(4), , pp.553-565
Main Authors: Kwon, Yoon Kyung, Choi, Soo Jung, Kim, Cho Rong, Kim, Jae Kyeom, Kim, Youn-Jung, Choi, Jong Hun, Song, Sang-Wook, Kim, Chang-Ju, Park, Gwi Gun, Park, Cheung-Seog, Shin, Dong-Hoon
Format: Article
Language:English
Subjects:
Alzheimer's disease
Antioxidants
Applied Microbiology
Bioactive compounds
Biological activity
Biological Techniques
Bioorganic Chemistry
Cell death
Chemistry
Chemistry and Materials Science
Chromatography
Cognitive ability
Column chromatography
Ethanol
High performance liquid chromatography
Hydrogen peroxide
Ionization
Lipid peroxidation
Lipids
Liquid chromatography
Malondialdehyde
Mass spectrometry
Mass spectroscopy
Memory
Neurodegenerative diseases
NMR
Nuclear magnetic resonance
Oxidation
Oxidative stress
Peroxidation
Pheochromocytoma cells
Quinic acid
Roots
농학
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Summary:Many studies have shown that oxidative stress induced by hydrogen peroxide can lead to neuronal cell death. In this study, protective effects of the ethanol extract of A. lappa L. roots against oxidative stress in PC12 cells were measured by 2′, 7′-dichlorofluorescein diacetate and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays. Oxidative stress contributes to the memory deficits induced by Beta-amyloid 1-42 in mice. Treatment with the ethanol extract of A. lappa L. roots improved working and reference memory in mice in the Y-maze and passive avoidance tests. To detect the lipid peroxidation levels, malondialdehyde was measured. To purify the bioactive compound, active fractions and components of A. lappa L. roots were isolated by partitioning, open column chromatography, TLC, high-performance liquid chromatography, electron ionization mass spectrometry, and 1 H/ 13 C-nuclear magnetic resonance. We identified the bioactive compound as quinic acid, which is a powerful antioxidant agent. Ultimately, the roots of A. lappa L. may become available as an antioxidant food for Alzheimer’s disease patients and those with other oxidative stress-induced disorders.
ISSN:2468-0834
2468-0842
DOI:10.1007/s13765-016-0195-2