Hair multiplication with dermal papilla like tissue containing human dermal papilla cells

Alopecia is not a critical disease; however it is a disease that can affect the quality of life. Many remedies have been developed to cure alopecia, but only two have been approved by the FDA. Due to the steadily increasing number of young alopecia patients, the need for new therapies for curing alo...

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Bibliographic Details
Published in:Biotechnology and bioprocess engineering 2014, 19(3), , pp.394-403
Main Authors: Yoo, Bo-Young, Shin, Youn-Ho, Yoon, Hee-Hoon, Seo, Young-Kwon, Park, Jung-Keug
Format: Article
Language:English
Subjects:
Alopecia
alternative medicine
Baldness
Biomedical research
Biotechnology
Chemistry
Chemistry and Materials Science
Collagen
Cytokines
drugs
epithelial cells
Follicles
Growth factors
Hair
Hair analysis
hair follicles
humans
in vitro studies
Industrial and Production Engineering
mice
morphogenesis
patients
Penicillin
Quality of life
Research Paper
Stem cells
Studies
toxicity
생물공학
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Summary:Alopecia is not a critical disease; however it is a disease that can affect the quality of life. Many remedies have been developed to cure alopecia, but only two have been approved by the FDA. Due to the steadily increasing number of young alopecia patients, the need for new therapies for curing alopecia is very high. Recent studies on cell therapy have reported using technique to treat various diseases. We introduce upgraded hair cell therapy which tested hair structure inducing activity with bioartificial dermal papilla tissue. Hair follicles contain two types of stem cells: Outer root sheath cells (ORSCs) derived epithelial cells, and dermal cells (DPCs). In this study, we reconstructed DP-like tissues (DPLTs) using cultured dermal papilla cells (DPCs) from human hair follicles. The DPLTs were produced special media (Dermal Papilla Forming Media: DPFM) conditions in vitro, which can induce epithelial stands from implanted healthy hair without DP. We tested in vivo hair-inducing with a modified hair sandwich model. Two to three weeks DPLT injection into the mouse scalp skin, we observed new hair in the injected site and detected injected human cells from DPLTs and Outer Root Sheath Cells (ORSCs) in the new hair via human Alu-DNA-specific probe. In the future, reconstructed DPLTs may be used in in vitro studies of hair development and the morphogenesis mechanism, as well as in vitro studies of the efficacy and toxicity of drugs for baldness. These tissues will be used as an alternative medicine product for hair transplantation
ISSN:1226-8372
1976-3816
DOI:10.1007/s12257-013-0632-6