Efficient immobilization technique for enhancement of cellobiose dehydrogenase activity on silica gel

In this study, cellobiose dehydrogenase (CDH) of Phanerochaete chrysosporium ATCC 32629 was immobilized on silica gel for the further application of CDH in the saccharification process of biomass. To prevent the loss of enzyme activity during enzyme immobilization, the pretreatment of CDH was perfor...

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Bibliographic Details
Published in:Biotechnology and bioprocess engineering 2012, 17(1), , pp.55-59
Main Authors: Kim, E.J., Korea University, Seoul, Republic of Korea, Song, Y.S., Korea University, Seoul, Republic of Korea, Choi, H.S., Korea University, Seoul, Republic of Korea, Yoo, H.Y., Korea University, Seoul, Republic of Korea, Kang, S.W., Korea University, Seoul, Republic of Korea, Song, K.H., Korea University, Seoul, Republic of Korea, Han, S.O., Korea University, Seoul, Republic of Korea, Kim, S.W., Korea University, Seoul, Republic of Korea
Format: Article
Language:English
Subjects:
Biodegradation
Bioengineering
Biomass
Biotechnology
cellobiose
cellobiose dehydrogenase
Cellulose
Chemistry
Chemistry and Materials Science
Cytochrome
Dehydrogenase
Dehydrogenases
Enzymatic activity
Enzymes
Fungi
IMMOBILISATION
IMMOBILIZATION
Industrial and Production Engineering
INMOVILIZACION
Lactose
Lignin
PHANEROCHAETE CHRYSOSPORIUM
pretreatment
Proteins
Research Paper
Silica
silica gel
Studies
white-rot fungi
Wood products
생물공학
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Summary:In this study, cellobiose dehydrogenase (CDH) of Phanerochaete chrysosporium ATCC 32629 was immobilized on silica gel for the further application of CDH in the saccharification process of biomass. To prevent the loss of enzyme activity during enzyme immobilization, the pretreatment of CDH was performed by various pretreatment materials before immobilization. When pretreated enzymes were used in immobilization, the activities of immobilized CDH were higher than non-pretreated CDH even in same amounts of immobilized protein. The specific activity of pretreated immobilized CDH with lactose was about two times higher than that of non-pretreated immobilized CDH. Moreover, the pretreated immobilized CDH showed better reusability than non-pretreated immobilized CDH, with 67.3% of its original activity being retained after 9 reuses.
ISSN:1226-8372
1976-3816
DOI:10.1007/s12257-011-0085-8