Quantitative Studies of Carbohydrate-protein Interaction using Functionalized Bacterial Spores in Solution and on Chips

Carbohydrate-protein interaction is one of the most important molecular events deemed critical for numerous biological processes. Therefore, understanding this interaction is essential. In this study, we used bacterial spore display techniques to present multiple copies of streptavidin on the surfac...

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Bibliographic Details
Published in:Biotechnology and bioprocess engineering 2011, 16(1), , pp.190-195
Main Authors: Park, T.J., Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea, Park, J.P., Daegu Haany University, Gyeongsan, Republic of Korea, Lee, S.J., Center for Nanobio Integration and Convergence Engineering (NICE), National Nanofab Center, Daejeon, Republic of Korea, Lee, S.Y., Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea
Format: Article
Language:English
Subjects:
Analysis
Bacteria
bacterial spore display
Bioengineering
Biotechnology
carbohydrate-protein interaction
Carbohydrates
Chemistry
Chemistry and Materials Science
Cloning
Concanavalin A
Drugs
Engineering
Enzymes
high-throughput screening
immobilization
Industrial and Production Engineering
LECTINAS
LECTINE
LECTINS
Ligands
Plasmids
Proteins
Quantitative research
Research Paper
Spores
streptavidin
Studies
생물공학
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Summary:Carbohydrate-protein interaction is one of the most important molecular events deemed critical for numerous biological processes. Therefore, understanding this interaction is essential. In this study, we used bacterial spore display techniques to present multiple copies of streptavidin on the surface of spores to explore carbohydrate-protein interaction in solution and on chips. By applying bacterial spores displaying streptavidin, we developed a new method which allows sensitive, versatile, and passive detection of carbohydrate-protein interactions with a 10-fold increase in sensitivity. The linear relationship of interactions between carbohydrates and labeled concanavalin A (con A) in solution and on functionalized bacterial spore chips has also been confirmed. To the best of our knowledge, this is the first example of development and characterization of binding behavior in carbohydrate-protein interactions using bacterial spore-displayed streptavidin. We believe this strategy may enable new high-throughput screening of carbohydrate interactions as well as establish a basis for monitoring inhibitors of carbohydrate-binding proteins when developing new drugs.
ISSN:1226-8372
1976-3816
DOI:10.1007/s12257-010-0063-6