Effect of Cultivation Conditions on Trehalose Content and Viability of Brewing Yeast Following Preservation via Filter Paper or Lyophilization Methods

The effects of variations in cultivation conditions on trehalose concentration and the viability of brewing yeasts following preservation by filter paper or lyophilization methods were evaluated. In case of filter paper preservation, the cultivation period had no affect on yeast viability, while agi...

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Bibliographic Details
Published in:Biotechnology and bioprocess engineering 2008, 13(6), , pp.690-696
Main Authors: Cheong, C. (Seoul University of Venture and Information, Seoul, Republic of Korea), Wackerbauer, Karl (Technical University of Berlin, Berlin, Germany), Beckmann, Martin (Technical University of Berlin, Berlin, Germany), Jang, K.H. (Kangwon National University, Samcheok, Republic of Korea), Kang, S.A. (Seoul University of Venture and Information, Seoul, Republic of Korea), E-mail: sakang@suv.ac.kr
Format: Article
Language:English
Subjects:
Biotechnology
brewing yeast
Chemistry
Chemistry and Materials Science
Chromatography
Cultivation
Fermentation
filter paper preservation
Industrial and Production Engineering
Metabolism
Methods
Nitrogen
Studies
TREHALOSA
TREHALOSE
VIABILIDAD
VIABILITE
VIABILITY
Yeast
Yeasts
생물공학
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Summary:The effects of variations in cultivation conditions on trehalose concentration and the viability of brewing yeasts following preservation by filter paper or lyophilization methods were evaluated. In case of filter paper preservation, the cultivation period had no affect on yeast viability, while agitation and aeration during cultivation had a positive effect regarding viability of the bottom-fermenting strains, Rh and Frank. For effective preservation, it was necessary to harvest yeast cells from the stationary phase during cultivation. For lyophilization preservation, the yeast strains tested showed a negative effect on viability, independent of strain or cultivation method. No significant correlation was found between trehalose concentration and yeast viability following either filter paper or lyophilization preservation. However, the filter paper preservation method was suitable for both bottom and top brewing yeast strains with regard to feasibility, viability, and maintenance of the yeast's specific character.
ISSN:1226-8372
1976-3816
DOI:10.1007/s12257-008-0066-8