Early diagnosis of radiodermatitis using lactate dehydrogenase isozymes in hairless mice (SKH1-hr)

• Published in: Laboratory animal research 2012, 28(4), , pp.239-244
• Main Authors: Cho, S.K., Cheongju University, Cheongju, Republic of Korea, Kim, W.D., Chungbuk National University, Cheongju, Republic of Korea
• Format: Article
• Language: English
• Subjects: hairless mice; ISOENZIMAS; ISOENZYME; ISOENZYMES; lactate dehydrogenase; Original; Radiodermatitis; 수의학
• ISSN: 1738-6055; 2233-7660
• DOI: 10.5625/lar.2012.28.4.239

In this study, we evaluate a method for the early diagnosis of radiodermatitis for use in the prevention and therapy of this condition. Hairless mice (SKH1-hr) were used to study the early diagnosis of radiodermatitis. Lactate dehydrogenase (LDH, EC 1.1.1.27) isozymes were analyzed using native-polyacrylamide gel electrophoresis and western blotting of blood serum and tissues collected from SKH1- hr mice. Radiodermatitis developed 24 days after the first X-irradiation. Reduced spleen weight was observed after the last X-irradiation (P less than 0.05). Thereafter the weight increased until 24 days after the first irradiation, finally reaching levels comparable to those in the sham-irradiated control group. LDH activity was the highest in skeletal muscle and lowest in blood serum. LDH C₄, A₄, A₃B, A₂B₂, AB₃, and B₄ isozymes were detected, in the mentioned order, from the cathode. This result was similar in other mouse strains. In the irradiated group, LDH A₄ isozyme levels were reduced in the serum until inflammation occurred, whereas those of B₄ isozyme were elevated. The subunits A and B followed a similar trend to that of LDH A₄ and B₄ isozyme, respectively. Importantly, antibodies against LDH B₄ isozyme could prove useful in the early diagnosis of radiodermatitis.