Mitochondrial activity in illuminated leaves of chlorophyll-deficient mutant rice (OsCHLH) seedlings

The rice CHLH gene encodes the Mg 2+ -chelatase H subunit, which is involved in chlorophyll biosynthesis. Growth of the chlorophyll-deficient oschlh mutant is supported by mitochondrial activity. In this study, we investigated the activity of mitochondrial respiration in the illuminated leaves durin...

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Published in:Plant biotechnology reports 2010, 4(4), , pp.281-291
Main Authors: Goh, Chang-Hyo, Satoh, Kouji, Kikuchi, Shoshi, Kim, Seong-Cheol, Ko, Suk-Min, Kang, Hong-Gyu, Jeon, Jong-Seong, Kim, Cheol Soo, Park, Youn-Il
Format: Article
Language:English
Subjects:
Acids
Agricultural biotechnology
Agriculture
Aquatic plants
ATP
Biomedical and Life Sciences
Biosynthesis
Biotechnology
Cell Biology
Chlorophyll
Dehydrogenases
Enzymes
Genes
Hydrogen peroxide
Kinases
Leaves
Life Sciences
Light
Metabolism
Mitochondria
Original Article
Oxidative stress
Photosynthesis
Plant Biochemistry
Plant propagation
Plant Sciences
Research centers
Respiration
Rice
Seedlings
Studies
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Summary:The rice CHLH gene encodes the Mg 2+ -chelatase H subunit, which is involved in chlorophyll biosynthesis. Growth of the chlorophyll-deficient oschlh mutant is supported by mitochondrial activity. In this study, we investigated the activity of mitochondrial respiration in the illuminated leaves during oschlh seedling development. Growth of mutant plants was enhanced in the presence of 3% sucrose, which may be used by mitochondria to meet cellular energy requirements. ATP content in these mutants was, however, significantly lowered in light conditions. Low cytosolic levels of NADH in illuminated oschlh mutant leaves further indicated the inhibition of mitochondrial metabolism. This down-regulation was particularly evident for oxidative stress-responsive genes in the mutant under light conditions. Hydrogen peroxide levels were higher in oschlh mutant leaves than in wild-type leaves; this increase was largely caused by the impairment of the expression of the antioxidant genes, such as OsAPX1 , OsRAC1 , and OsAOXc in knockout plants. Moreover, treatment of mesophyll protoplasts with ascorbic acid or catalase recovered ATP content in the mutants. Taken together, these results suggest that the light-mediated inhibition of mitochondrial activity leads to stunted growth of CHLH rice seedlings.
ISSN:1863-5466
1863-5474
DOI:10.1007/s11816-010-0146-z