Promotive effects of human induced pluripotent stem cell-conditioned medium on the proliferation and migration of dermal fibroblasts

Fibroblasts are a major cell type in the dermis. When skin is wounded in various ways such as by abrasions, cuts or diabetic ulcer, proliferation and migration of dermal fibroblasts is necessary for cutaneous wound healing. Numerous studies have shown that adult stem cells secrete paracrine factors...

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Bibliographic Details
Published in:Biotechnology and bioprocess engineering 2017, 22(5), , pp.561-568
Main Authors: Oh, Myeongsik, Kim, Yu Jin, Son, Young Ju, Yoo, Hyuk Sang, Park, Ju Hyun
Format: Article
Language:English
Subjects:
Biotechnology
Cell proliferation
Cell self-renewal
Chemistry
Chemistry and Materials Science
Conditioning
Dermis
Diabetes mellitus
Dilution
Effector cells
Embryo fibroblasts
Fibroblasts
Industrial and Production Engineering
Paracrine signalling
Pluripotency
Research Paper
Rodents
Skin
Stem cell transplantation
Stem cells
Wound healing
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Summary:Fibroblasts are a major cell type in the dermis. When skin is wounded in various ways such as by abrasions, cuts or diabetic ulcer, proliferation and migration of dermal fibroblasts is necessary for cutaneous wound healing. Numerous studies have shown that adult stem cells secrete paracrine factors and these are able to promote wound healing by activating migration and proliferation of effector cells such as dermal fibroblasts. However, the paracrine factors secreted from pluripotent stem cells and the effect of these on dermal fibroblast proliferation and migration have been poorly characterized. In this study we cultured human induced pluripotent stem cells without any animal-derived components including feeder cells, and investigated the effect of stem cell-conditioned medium (iPSC-CM) on dermal fibroblast proliferation and migration. Results showed that the proliferation of mouse embryonic fibroblasts (STO cells) and human dermal fibroblasts (HDFs) were significantly stimulated by iPSC-CM. We determined that the optimal concentration of iPSC-CM in promoting the proliferation of HDFs was a 75% dilution. Scratch wound assay and transwell migration assay also demonstrated the stimulatory effect of iPSC-CM on the migration of HDFs. iPSC-CM is believed to have advantages because of the unique capabilities of iPSCs, which include infinite self-renewal, pluripotency and variety of donor cells. Thus, iPSC-CM is anticipated to be a valuable source of paracrine factors which can potentially be used for wound healing applications.
ISSN:1226-8372
1976-3816
DOI:10.1007/s12257-017-0221-1