Transplantation of a Scaffold-Free Cartilage Tissue Analogue for the Treatment of Physeal Cartilage Injury of the Proximal Tibia in Rabbits

The purpose of this study was to investigate the effects of transplantation of an in vitro-generated, scaffold-free, tissue-engineered cartilage tissue analogue (CTA) using a suspension chondrocyte culture in a rabbit growth-arrest model. We harvested cartilage cells from the articular cartilage of...

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Bibliographic Details
Published in:Yonsei medical journal 2016, 57(2), , pp.441-448
Main Authors: Lee, Sang Uk, Lee, Jae Young, Joo, Sun Young, Lee, Yong Suk, Jeong, Changhoon
Format: Article
Language:English
Subjects:
Animals
Bone Transplantation
Cartilage - anatomy & histology
Cell Culture Techniques
Cells, Cultured
Chondrocytes - cytology
Chondrocytes - transplantation
Growth Plate - anatomy & histology
Growth Plate - surgery
Mesenchymal Stem Cell Transplantation
Original
Rabbits
Tibia - surgery
Tissue Engineering
Transplantation, Autologous - methods
Transplantation, Homologous
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Summary:The purpose of this study was to investigate the effects of transplantation of an in vitro-generated, scaffold-free, tissue-engineered cartilage tissue analogue (CTA) using a suspension chondrocyte culture in a rabbit growth-arrest model. We harvested cartilage cells from the articular cartilage of the joints of white rabbits and made a CTA using a suspension culture of 2×10⁷ cells/mL. An animal growth plate defect model was made on the medial side of the proximal tibial growth plate of both tibias of 6-week-old New Zealand white rabbits (n=10). The allogenic CTA was then transplanted onto the right proximal tibial defect. As a control, no implantation was performed on the left-side defect. Plain radiographs and the medial proximal tibial angle were obtained at 1-week intervals for evaluation of bone bridge formation and the degree of angular deformity until postoperative week 6. We performed a histological evaluation using hematoxylin-eosin and Alcian blue staining at postoperative weeks 4 and 6. Radiologic study revealed a median medial proximal tibial angle of 59.0° in the control group and 80.0° in the CTA group at 6 weeks. In the control group, statistically significant angular deformities were seen 3 weeks after transplantation (p
ISSN:0513-5796
1976-2437
DOI:10.3349/ymj.2016.57.2.441