Bone Marrow Mononuclear Stem Cells Transplanted in Rat Infarct Myocardium Improved the Electrical Conduction without Evidence of Proarrhythmic Effects

• Published in: Yonsei medical journal 2007, 48(5), , pp.754-764
• Main Authors: 정보영, 김일권, 이문형, 유경종, 김성순
• Format: Article
• Language: Korean
• Subjects: 의학일반
• ISSN: 0513-5796; 1976-2437

Purpose: The arrhythmogenic effect of stem cells transplantation (SCT) in an infarct myocardium is still unknown. We investigated arrhythmogenicity of SCT in rat cryo-infarct model. Materials and Methods: In rat cryo-infarct model, bone marrow mononuclear stem cells (MNSC, 1×107 cells) were transplanted into the infarct border zone (BZ) of the LV epicardium. We compared the optical mapping and inducibility of ventricular tachycardia/fibrillation (VT/VF) among normal (n=5), cryo-infarct (n=6), and SCT rats (n=6). Results: The VT/VF inducibility was higher in the cryo- infarct (47.2%, p=0.001) and SCT groups (34.6%, p=0.01) than in the normal group (12.8%). The induced VT/VF episodes persisted for more than 2 minutes in 4.3%, 26.4% and 17.3% in the normal, cryo-infarct and SCT group, respectively. In the SCT group, the action potential duration at 70% was shorter at the SCT site than the BZ during SR (75.2±8.1 vs. 145.6±4.4 ms, p=0.001) and VT (78.2± 13.0 vs. 125.7±21.0 ms, p= 0.001). Conduction block was observed at the SCT site and BZ during VT. However, no reentry or ectopic foci were observed around the SCT sites. Conclusion: The electrical conduction was improved by SCT without evidence of augmentation of arrhythmia in the rat cryo-infarct model. KCI Citation Count: 1