Unilateral ureteral obstruction causes gut microbial dysbiosis and metabolome disorders contributing to tubulointerstitial fibrosis

• Published in: Experimental & molecular medicine 2019, 51(0), , pp.1-18
• Main Authors: Chen, Lin, Chen, Dan-Qian, Liu, Jing-Ru, Zhang, Jun, Vaziri, Nosratola D., Zhuang, Shougang, Chen, Hua, Feng, Ya-Long, Guo, Yan, Zhao, Ying-Yong
• Format: Article
• Language: English
• Subjects: 101/58; 38/39; 45/47; 631/208/325; 692/699/1585; 82/16; 82/51; 82/80; Amino acids; Animals; Atrophy; Biomedical and Life Sciences; Biomedicine; Cardiovascular diseases; Dysbacteriosis; Dysbiosis - etiology; Dysbiosis - metabolism; Dysbiosis - microbiology; Dysbiosis - pathology; Fibrosis; Gastrointestinal Microbiome; Inflammation; Intestinal microflora; Kidney diseases; Kidney Diseases - etiology; Kidney Diseases - metabolism; Kidney Diseases - microbiology; Kidney Diseases - pathology; Kidney Tubules - pathology; Male; Medical Biochemistry; Metabolic Networks and Pathways; Metabolism; Metabolites; Metabolome; Metabolomics; Microbiomes; Microbiota; Molecular Medicine; Morbidity; Rats, Sprague-Dawley; Rodents; rRNA 16S; Serotonin; Stem Cells; Tryptophan 2,3-dioxygenase; Tryptophan synthase; Ureteral Obstruction - complications; Ureteral Obstruction - metabolism; Ureteral Obstruction - microbiology; Ureteral Obstruction - pathology; 생화학
• ISSN: 1226-3613; 2092-6413
• DOI: 10.1038/s12276-019-0234-2

Chronic kidney disease (CKD) increases the risk and prevalence of cardiovascular disease (CVD) morbidity and mortality. Recent studies have revealed marked changes in the composition of the microbiome and the metabolome and their potential influence in renal disease and CVD via the accumulation of microbial-derived uremic toxins. However, the effect of unilateral ureteral obstruction (UUO) on the gut microbiome and circulating metabolites is unknown. Male Sprague-Dawley rats were randomized to UUO and sham-operated control groups. Renal histology, colonic microbiota, and plasma metabolites were examined two weeks later. We employed 16S rRNA sequence and untargeted metabolomic analyses to explore the changes in colonic microbiota and plasma metabolites and their relationship with tubulointerstitial fibrosis (TIF). The UUO rats exhibited tubular atrophy and dilatation, interstitial fibrosis and inflammatory cell infiltration in the obstructed kidney. UUO rats showed significant colonic enrichment and depletion of genera. Significant differences were identified in 219 plasma metabolites involved in lipid, amino acid, and bile acid metabolism, which were consistent with gut microbiota-related metabolism. Interestingly, tryptophan and its metabolites kynurenine, 5-hydroxytryptophan and 5-hydroxytryptamine levels, which were linked with TIF, correlated with nine specific genera. Plasma tryptophan level was positively correlated with Clostridium IV, Turicibacter , Pseudomonas and Lactobacillales , and negatively correlated with Oscillibacter , Blautia , and Intestinimonas , which possess the genes encoding tryptophan synthase (K16187), indoleamine 2,3-dioxygenase (K00463) and tryptophan 2,3-dioxygenase (K00453) and their corresponding enzymes (EC:1.13.11.52 and EC:1.13.11.11) that exacerbate TIF. In conclusion, UUO results in profound changes in the gut microbiome and circulating metabolites, events that contribute to the pathogenesis of inflammation and TIF. Chronic kidney disease: The contribution of gut bacteria An imbalance in gut bacteria contributes to kidney tissue scarring and declined kidney function. An international study led by Ying-Yong Zhao at Northwest University, Xi’an, China, analyzed the composition of the gut microbes in a rat model of chronic renal injury. They found that urinary tract obstruction was associated with changes in gut microbe composition and altered gut microbe-related metabolism of lipids, amino acids and bile acid. Lower levels