Lipoteichoic Acid Isolated from Lactobacillus plantarum Inhibits Melanogenesis in B16F10 Mouse Melanoma Cells

• Published in: Molecules and cells 2015, 38(2), , pp.163-170
• Main Authors: Kim, H.R., Kyung Hee University, Yongin, Republic of Korea, Kim, H., Kyung Hee University, Yongin, Republic of Korea, Jung, B.J., Kyung Hee University, Yongin, Republic of Korea, You, G.E., Kyung Hee University, Yongin, Republic of Korea, Jang, S., Institute Pasteur Korea, Seongnam, Republic of Korea, Chung, D.K., Kyung Hee University, Yongin, Republic of Korea
• Format: Article
• Language: English
• Subjects: Animals; Cell Line, Tumor; Cell Proliferation - drug effects; Cells; Gene Expression Regulation, Neoplastic - drug effects; LACTOBACILLUS PLANTARUM; Lactobacillus plantarum - chemistry; Lipopolysaccharides - pharmacology; lipoteichoic acid,melanogenesis,microphthalmia-associated transcription factor (MITF),RNA stability; MAP Kinase Signaling System - drug effects; Melanoma - enzymology; Melanoma - pathology; Mice; Microphthalmia-Associated Transcription Factor - genetics; Microphthalmia-Associated Transcription Factor - metabolism; Teichoic Acids - pharmacology; 생물학
• ISSN: 1016-8478; 0219-1032
• DOI: 10.14348/molcells.2015.2263

Lipoteichoic acid (LTA) is a major component of the cell wall of Gram-positive bacteria. Its effects on living organisms are different from those of lipopolysaccharide (LPS) found in Gram-negative bacteria. LTA contributes to immune regulatory effects including anti-aging. In this study, we showed that LTA isolated from Lactobacillus plantarum (pLTA) inhibited melanogenesis in B16F10 mouse melanoma cells. pLTA reduced the cellular activity of tyrosinase and the expression of tyrosinase family members in a dose-dependent manner. The expression of microphthalmia- associated transcription factor (MITF), a key factor in the synthesis of melanin, was also decreased by pLTA. Further, we showed that pLTA activated melanogenesis signaling, such as extracellular signal-regulated kinase (ERK) and phosphatidylinositol 3-kinse (PI3K)/AKT. In addition, the expression of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) and HuR, which are important RNA-binding proteins (RBPs), was reduced. pLTA likely degrades MITF via regulation of melanogenic signaling and RNA stability of melanogenic proteins, resulting in the reduction of melanin. Thus, our data suggest that pLTA has therapeutic potential for treating hyperpigmentation disorders and can also be used as a cosmetic whitening agent.