Pyrosequencing-based analysis of fecal microbial communities in three purebred pig lines

This study examined the fecal bacterial diversity of 15-weekold pigs from three purebred lines: Duroc, Landrace, and Yorkshire. Taxon-dependent and -independent analyses were performed to evaluate differences in the fecal bacterial communities and to identify bacterial genera that can be used to dis...

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Bibliographic Details
Published in:The journal of microbiology 2014, 52(8), , pp.646-651
Main Authors: Pajarillo, Edward Alain B., Chae, Jong Pyo, Balolong, Marilen P., Kim, Hyeun Bum, Seo, Kang-Seok, Kang, Dae-Kyung
Format: Article
Language:English
Subjects:
Animals
Antibiotics
Biomedical and Life Sciences
Breeding
Clostridium
Community composition
Discriminant analysis
Feces
Feces - microbiology
Feeds
Genes, rRNA
Genetic Variation
Hogs
Life Sciences
Microbial activity
Microbial Ecology and Environmental Microbiology
Microbiology
Microbiota
Prevotella
Principal Component Analysis
RNA, Ribosomal, 16S - genetics
Sequence Analysis, DNA - methods
Sus scrofa - microbiology
Swine
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Summary:This study examined the fecal bacterial diversity of 15-weekold pigs from three purebred lines: Duroc, Landrace, and Yorkshire. Taxon-dependent and -independent analyses were performed to evaluate differences in the fecal bacterial communities and to identify bacterial genera that can be used to discriminate breeds, following high-throughput pyrosequencing of 16S rRNA genes. Among the breeds evaluated, Landrace had the most diverse bacterial community composition. Prevotella , Blautia , Oscillibacter , and Clostridium were detected in all samples regardless of breed. On the other hand, Catenibacterium , Blautia , Dialister , and Sphaerochaeta were differentially detected among breeds, as demonstrated by the canonical loading plot. The discriminant analysis of principal components plot also showed clear separation of the three purebred pig lines, with a certain degree of similarity between Landrace and Yorkshire pigs and a distinct separation between Duroc pigs and the other two breeds. Other factors not related to breed, such as season or time of sampling and pen effects, may contribute to shaping the gut microbiota of pigs.
ISSN:1225-8873
1976-3794
DOI:10.1007/s12275-014-4270-2