Cellobiose dehydrogenase from the agaricomycete Coprinellus aureogranulatus and its application for the synergistic conversion of rice straw

From the biotechnological viewpoint, the enzymatic disintegration of plant lignocellulosic biomass is a promising goal since it would deliver fermentable sugars for the chemical sector. Cellobiose dehydrogenase (CDH) is a vital component of the extracellular lignocellulose-degrading enzyme system of...

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Published in:Applied biological chemistry 2021, 64(5), , pp.1-11
Main Authors: Nghi, Do Huu, Kellner, Harald, Büttner, Enrico, Huong, Le Mai, Duy, Le Xuan, Giap, Vu Dinh, Quynh, Dang Thu, Hang, Tran Thi Nhu, Verberckmoes, An, Diels, Ludo, Liers, Christiane, Hofrichter, Martin
Format: Article
Language:English
Subjects:
Applied Microbiology
Biodegradation
Biological Techniques
Biomass
Bioorganic Chemistry
Catalysts
Cellobiose
Cellobiose dehydrogenase
Cellulase
Chemistry
Chemistry and Materials Science
Conversion
Dehydrogenase
Dehydrogenases
Disintegration
Enzymes
Esterase
Gluconic acid
Lignocellulose
Metabolites
Oligosaccharides
Optimization
Rice straw
Straw
Sugar
Xylanase
농학
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Summary:From the biotechnological viewpoint, the enzymatic disintegration of plant lignocellulosic biomass is a promising goal since it would deliver fermentable sugars for the chemical sector. Cellobiose dehydrogenase (CDH) is a vital component of the extracellular lignocellulose-degrading enzyme system of fungi and has a great potential to improve catalyst efficiency for biomass processing. In the present study, a CDH from a newly isolated strain of the agaricomycete Coprinellus aureogranulatus ( Cau CDH) was successfully purified with a specific activity of 28.9 U mg −1 . This pure enzyme (MW = 109 kDa, p I = 5.4) displayed the high oxidative activity towards β-1–4-linked oligosaccharides. Not least, Cau CDH was used for the enzymatic degradation of rice straw without chemical pretreatment. As main metabolites, glucose (up to 165.18 ± 3.19 mg g −1 ), xylose (64.21 ± 1.22 mg g −1 ), and gluconic acid (5.17 ± 0.13 mg g −1 ) could be identified during the synergistic conversion of this raw material with the fungal hydrolases (e.g., esterase, cellulase, and xylanase) and further optimization by using an RSM statistical approach.
ISSN:2468-0834
2468-0842
DOI:10.1186/s13765-021-00637-y