Liquid Microjunction Surface Sampling Probe Fluid Dynamics: Computational and Experimental Analysis of Coaxial Intercapillary Positioning Effects on Sample Manipulation

A coaxial geometry liquid microjunction surface sampling probe (LMJ-SSP) enables direct extraction of analytes from surfaces for subsequent analysis by techniques like mass spectrometry. Solution dynamics at the probe-to-sample surface interface in the LMJ-SSP has been suspected to influence samplin...

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Bibliographic Details
Published in:Journal of the American Society for Mass Spectrometry 2011-07, Vol.22 (7), p.1157-1166, Article s13361-011-0145-5
Main Authors: ElNaggar, Mariam S., Barbier, Charlotte, Van Berkel, Gary J.
Format: Article
Language:English
Subjects:
Analytical Chemistry
Bioinformatics
Biotechnology
CAPILLARIES
Chemistry
Chemistry and Materials Science
CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS
Coloring Agents - chemistry
Computational fluid dynamics
Computer Simulation
COMPUTERIZED SIMULATION
EDDY CURRENTS
EFFICIENCY
Electrospray ionization
Experimentation
Fluid dynamics
FLUID MECHANICS
GEOMETRY
Hydrodynamics
IMPLEMENTATION
MASS SPECTROMETERS
Mass spectrometry
MASS SPECTROSCOPY
Models, Chemical
Organic Chemistry
POSITIONING
PROBES
Proteomics
Research Article
SAMPLING
Spectrometry, Mass, Electrospray Ionization - instrumentation
Spectrometry, Mass, Electrospray Ionization - methods
Surface sampling
Surface sampling probe
TRANSPORT
Visual observation
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Description
Summary:A coaxial geometry liquid microjunction surface sampling probe (LMJ-SSP) enables direct extraction of analytes from surfaces for subsequent analysis by techniques like mass spectrometry. Solution dynamics at the probe-to-sample surface interface in the LMJ-SSP has been suspected to influence sampling efficiency and dispersion but has not been rigorously investigated. The effect on flow dynamics and analyte transport to the mass spectrometer caused by coaxial retraction of the inner and outer capillaries from each other and the surface during sampling with a LMJ-SSP was investigated using computational fluid dynamics and experimentation. A transparent LMJ-SSP was constructed to provide the means for visual observation of the dynamics of the surface sampling process. Visual observation, computational fluid dynamics (CFD) analysis, and experimental results revealed that inner capillary axial retraction from the flush position relative to the outer capillary transitioned the probe from a continuous sampling and injection mode through an intermediate regime to sample plug formation mode caused by eddy currents at the sampling end of the probe. The potential for analytical implementation of these newly discovered probe operational modes is discussed.
ISSN:1044-0305
1879-1123
DOI:10.1007/s13361-011-0145-5