16S rRNA Gene Microarray Analysis of Microbial Communities in Ethanol-Stimulated Subsurface Sediment

A high-density 16S rRNA gene microarray was used to analyze microbial communities in a slurry of ethanol-amended, uranium-contaminated subsurface sediment. Of specific interest was the extent to which the microarray could detect temporal patterns in the relative abundance of major metabolic groups (...

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Bibliographic Details
Published in:Microbes and Environments 2011, Vol.26(3), pp.261-265
Main Authors: Mohanty, Santosh R., Kollah, Bharati, Brodie, Eoin L., Hazen, Terry C., Roden, Eric E.
Format: Article
Language:English
Subjects:
16S rRNA gene
BASIC BIOLOGICAL SCIENCES
COMMUNITIES
DNA microarray
ENVIRONMENTAL SCIENCES
GENES
GEOSCIENCES
microbial community
SEDIMENTS
terminal electron accepting process
uranium reduction
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Summary:A high-density 16S rRNA gene microarray was used to analyze microbial communities in a slurry of ethanol-amended, uranium-contaminated subsurface sediment. Of specific interest was the extent to which the microarray could detect temporal patterns in the relative abundance of major metabolic groups (nitrate-reducing, metal-reducing, sulfate-reducing, and methanogenic taxa) that were stimulated by ethanol addition. The results show that the microarray, when used in conjunction with geochemical data and knowledge of the physiological properties of relevant taxa, provided accurate assessment of the response of key functional groups to biostimulation.
ISSN:1342-6311
1347-4405
DOI:10.1264/jsme2.ME11111