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Topological Effects on Globular Protein-ELP Fusion Block Copolymer Self-Assembly
Perfectly defined, monodisperse fusion protein block copolymers of a thermoresponsive coil‐like protein, ELP, and a globular protein, mCherry, are demonstrated to act as fully biosynthetic analogues to protein‐polymer conjugates that can self‐assemble into biofunctional nanostructures such as hexago...
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Published in: | Advanced functional materials 2015-02, Vol.25 (5), p.729-738 |
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Main Authors: | , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Perfectly defined, monodisperse fusion protein block copolymers of a thermoresponsive coil‐like protein, ELP, and a globular protein, mCherry, are demonstrated to act as fully biosynthetic analogues to protein‐polymer conjugates that can self‐assemble into biofunctional nanostructures such as hexagonal and lamellar phases in concentrated solutions. The phase behavior of two mCherry‐ELP fusions, E10‐mCherry‐E10 and E20‐mCherry, is investigated to compare linear and bola fusion self‐assembly both in diluted and concentrated aqueous solution. In dilute solution, the molecular topology impacts the stability of micelles formed above the thermal transition temperature of the ELP block, with the diblock forming micelles and the bola forming unstable aggregates. Despite the chemical similarity of the two protein blocks, the materials order into block copolymer‐like nanostructures across a wide range of concentrations at 30 wt% and above, with the bola fusion having a lower order‐disorder transition concentration than the diblock fusion. The topology of the molecule has a large impact on the type of nanostructure formed, with the two fusions forming phases in the opposite order as a function of temperature and concentration. This new system provides a rich landscape to explore the capabilities of fusion architecture to control supramolecular assemblies for bioactive materials.
Self‐assembly of fusion proteins containing a folded globular protein into solid nanomaterials is demonstrated for the first time. Using model molecules containing ELP and mCherry, the ability to tune nanostructure is demonstrated through control over the fusion topology. As fully biosynthetic analogues to protein‐polymer conjugates, the mCherry‐ELP fusions provide an efficient route to introduce complex biological functionality into supramolecular assemblies. |
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ISSN: | 1616-301X 1616-3028 |
DOI: | 10.1002/adfm.201403453 |