Blood group typing based on recording the elastic scattering of laser radiation using the method of digital imaging

The possibility is demonstrated to determine the human blood group by recording the scattering of laser radiation with the help of the digital imaging method. It is experimentally shown that the action of a standing ultrasound wave leads to acceleration of the agglutination reaction of red blood cel...

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Bibliographic Details
Published in:Quantum electronics (Woodbury, N.Y.) N.Y.), 2012-05, Vol.42 (5), p.409-416
Main Authors: Dolmashkin, A A, Dubrovskii, V A, Zabenkov, I V
Format: Article
Language:English
Subjects:
ANTIGEN-ANTIBODY REACTIONS
BIOPHYSICS
BLOOD CELLS
BLOOD GROUPS
CHARGES
CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS
ELASTIC SCATTERING
LASER RADIATION
LIGHT SCATTERING
MIXTURES
OPTIMIZATION
RESOLUTION
SAMPLE PREPARATION
SEDIMENTATION
SIMULATION
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Summary:The possibility is demonstrated to determine the human blood group by recording the scattering of laser radiation with the help of the digital imaging method. It is experimentally shown that the action of a standing ultrasound wave leads to acceleration of the agglutination reaction of red blood cells, to formation of larger immune complexes of red blood cells, and, as a consequence, to acceleration of their sedimentation. In the absence of agglutination of red blood cells the ultrasound does not enhance the relevant processes. This difference in the results of ultrasound action on the mixture of blood and serum allows a method of blood typing to be offered. Theoretical modelling of the technique of the practical blood typing, carried out on the basis of the elastic light scattering theory, agrees well with the experimental results, which made it possible to plan further improvement of the proposed method. The studies of specific features of sedimentation of red blood cells and their immune complexes were aimed at the optimisation of the sample preparation, i.e., at the search for such experimental conditions that provide the maximal resolution of the method and the device for registering the reaction of red blood cells agglutination. The results of the study may be used in designing the instrumentation for blood group assessment in humans.
ISSN:1063-7818
1468-4799
DOI:10.1070/QE2012v042n05ABEH014888