Actin cytoskeleton organization, cell surface modification and invasion rate of 5 glioblastoma cell lines differing in PTEN and p53 status

• Published in: Experimental cell research 2015-01, Vol.330 (2), p.346-357
• Main Authors: Djuzenova, Cholpon S., Fiedler, Vanessa, Memmel, Simon, Katzer, Astrid, Hartmann, Susanne, Krohne, Georg, Zimmermann, Heiko, Scholz, Claus-Jürgen, Polat, Bülent, Flentje, Michael, Sukhorukov, Vladimir L.
• Format: Article
• Language: English
• Subjects: 60 APPLIED LIFE SCIENCES; ACTIN; Actin Cytoskeleton; Actin remodeling; Actins - biosynthesis; Benzothiazoles - pharmacology; Brain cancer; Brain Neoplasms - pathology; Cell adhesion; Cell adhesion & migration; Cell Adhesion - genetics; Cell Line, Tumor; Cell matrix; Cell Movement - genetics; CYTOPLASM; Cytoskeleton; GENES; Genotype & phenotype; Glioblastoma - pathology; GLIOMAS; Humans; MAP Kinase Signaling System - genetics; Matrix-metalloprotease; MICROTUBULES; Mutation; Neoplasm Invasiveness - genetics; Neoplasm Invasiveness - pathology; Phosphatidylinositol 3-Kinases - biosynthesis; Proto-Oncogene Proteins c-akt - biosynthesis; PTEN Phosphohydrolase - genetics; PTEN Phosphohydrolase - metabolism; RNA Interference; RNA, Small Interfering; SCANNING ELECTRON MICROSCOPY; Stress fibers; Toluene - analogs & derivatives; Toluene - pharmacology; TOR Serine-Threonine Kinases - biosynthesis; TUMOR CELLS; Tumor Suppressor Protein p53 - antagonists & inhibitors; Tumor Suppressor Protein p53 - genetics; Tumor Suppressor Protein p53 - metabolism
• ISSN: 0014-4827; 1090-2422
• DOI: 10.1016/j.yexcr.2014.08.013

Glioblastoma cells exhibit highly invasive behavior whose mechanisms are not yet fully understood. The present study explores the relationship between the invasion capacity of 5 glioblastoma cell lines differing in p53 and PTEN status, expression of mTOR and several other marker proteins involved in cell invasion, actin cytoskeleton organization and cell morphology. We found that two glioblastoma lines mutated in both p53 and PTEN genes (U373-MG and SNB19) exhibited the highest invasion rates through the Matrigel or collagen matrix. In DK-MG (p53wt/PTENwt) and GaMG (p53mut/PTENwt) cells, F-actin mainly occurred in the numerous stress fibers spanning the cytoplasm, whereas U87-MG (p53wt/PTENmut), U373-MG and SNB19 (both p53mut/PTENmut) cells preferentially expressed F-actin in filopodia and lamellipodia. Scanning electron microscopy confirmed the abundant filopodia and lamellipodia in the PTEN mutated cell lines. Interestingly, the gene profiling analysis revealed two clusters of cell lines, corresponding to the most (U373-MG and SNB19, i.e. p53 and PTEN mutated cells) and less invasive phenotypes. The results of this study might shed new light on the mechanisms of glioblastoma invasion. •We examine 5 glioblastoma lines on the invasion capacity and actin cytoskeleton.•Glioblastoma cell lines mutated in both p53 and PTEN were the most invasive.•Less invasive cells showed much less lamellipodia, but more actin stress fibers.•A mechanism for the differences in tumor cell invasion is proposed.