Comparative Response of Indigenously Developed Bacterial Consortia on Progressive Degradation of Polyhydroxybutyrate Film Composites

• Published in: Journal of polymers and the environment 2018-07, Vol.26 (7), p.2661-2675
• Main Authors: Raghuwanshi, Shikha, Zaidi, M. G. H., Kumar, Saurabh, Goel, Reeta
• Format: Article
• Language: English
• Subjects: AIR; BACILLUS; Bacteria; Biodegradability; Biodegradable materials; BIODEGRADATION; Bioplastics; Chemistry; Chemistry and Materials Science; Consortia; COPOLYMERS; ECOSYSTEMS; Electron microscopy; Environmental Chemistry; Environmental conditions; Environmental Engineering/Biotechnology; ENVIRONMENTAL SCIENCES; FOURIER TRANSFORM SPECTROMETERS; Fourier transforms; INCUBATION; Industrial Chemistry/Chemical Engineering; Infrared analysis; Infrared spectroscopy; Lysinibacillus; Materials Science; Mixtures; MORPHOLOGY; Original Paper; Polyhydroxybutyrate; Polyhydroxybutyric acid; Polymer Sciences; PSEUDOMONAS; SCANNING ELECTRON MICROSCOPY; Soil conditions; Soil water; SOILS; WATER
• ISSN: 1566-2543; 1572-8919
• DOI: 10.1007/s10924-017-1159-2

The global demand of bioplastics has lead to an exponential increase in their production commercially. Hence, biodegradable nature needs to be evaluated in various ecosystems viz. air, water, soil and other environmental conditions to avoid the polymeric waste accumulation in the nature. In this paper, we investigated the progressive response of two indigenously developed bacterial consortia, i.e., consortium-I (C-I: Pseudomonas sp. strain Rb10, Pseudomonas sp. strain Rb11 and Bacillus sp. strain Rb18), and consortium-II (C-II: Lysinibacillus sp. strain Rb1, Pseudomonas sp. strain Rb13 and Pseudomonas sp. strain Rb19), against biodegradation behavior of polyhydroxybutyrate (PHB) film composites, under natural soil ecosystem (in net house). The biodegraded films recovered after 6 and 9 months of incubation were analyzed through Fourier transform infrared spectroscopy and scanning electron microscopy to determine the variations in chemical and morphological parameters (before and after incubation). Noticeable changes in the bond intensity, surface morphology and conductivity were found when PHB composites were treated with C-II. These changes were drastic in case of blends in comparison to copolymer. The potential isolates not only survived, but, also, there was a significant increase in bacterial diversity during whole period of incubation. To the best of our knowledge, it is the first report which described the biodegradation potential of Lysinibacillus sp. as a part of C-II with Pseudomonas sp. against PHB film composites.