Effect of Non-Thermal Plasma on Proliferative Activity and Adhesion of Multipotent Stromal Cells to Scaffolds Developed for Tissue-Engineered Constructs

We studied the effect of non-thermal argon plasma on proliferative activity of bone marrow multipotent stromal cells in vitro . Treatment of stromal cell suspension with pure argon did not affect their proliferation. The cells treated with non-thermal argon plasma and explanted in the treatment medi...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2019-05, Vol.167 (1), p.182-188
Main Authors: Chailakhyan, R. K., Grosheva, A. G., Gerasimov, Yu. V., Vorob’eva, N. N., Ermolaeva, S. A., Sysolyatina, E. V., Kazakova, M. V., Akishev, Yu. S., Petryakov, A. V., Sidoruk, K. V., Burdukovskii, V. F., Timashev, P. S.
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
Analysis
Animals
ARGON
Biomedical and Life Sciences
Biomedicine
BONE MARROW
Caprolactone
Cell Adhesion - drug effects
Cell Adhesion - physiology
Cell Biology
Cell Differentiation - drug effects
Cell Differentiation - physiology
CELL PROLIFERATION
Cell Proliferation - drug effects
Cell Proliferation - physiology
Cells, Cultured
CENTRIFUGATION
IN VITRO
Internal Medicine
Laboratory Medicine
Multipotent Stem Cells - cytology
Multipotent Stem Cells - drug effects
Pathology
Plasma
Plasma Gases - pharmacology
Rabbits
Stem cell research
Stromal cells
Tissue engineering
Tissue Engineering - methods
Tissue Scaffolds - chemistry
Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
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Summary:We studied the effect of non-thermal argon plasma on proliferative activity of bone marrow multipotent stromal cells in vitro . Treatment of stromal cell suspension with pure argon did not affect their proliferation. The cells treated with non-thermal argon plasma and explanted in the treatment medium demonstrated growth inhibition by 30-40% in comparison with the control. Multipotent stromal cells treated with plasma and after centrifugation explanted in normal medium within 12 min demonstrated accelerated growth. The total cell growth from the pellet and supernatant significantly exceeded the control values. We also analyzed adhesion and proliferative activity of multipotent stromal cells treated with non-thermal plasma on bioresorbable carriers. The cells adhered and proliferated on all types of studied samples. Adhesion properties of scaffolds differed. Caprolactone was found to be the most suitable material for adhesion and proliferation of multipotent stromal cells.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-019-04486-0