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Detection of mycobacterial small RNA in the bacterial culture supernatant and plasma of patients with active tuberculosis
Bacterial small RNA (sRNA) has been shown to play an important role in control of bacteria virulence, stress response and physiological metabolism by post-transcriptional regulation of gene expression. However, there were few reports about bacterial sRNA as a biomarker of infection. To test the pote...
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| Published in: | Biochemical and biophysical research communications 2018-09, Vol.503 (2), p.490-494 |
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| container_end_page | 494 |
| container_issue | 2 |
| container_start_page | 490 |
| container_title | Biochemical and biophysical research communications |
| container_volume | 503 |
| creator | Fu, Yingmei Li, Wenjing Wu, Zheng Tao, Yuanmeihui Wang, Xinyang Wei, Jing Jiang, Peidong Wu, Jinyin Zhang, Zhiwei Zhang, Wenli Zhao, Jizi Zhang, Fengmin |
| description | Bacterial small RNA (sRNA) has been shown to play an important role in control of bacteria virulence, stress response and physiological metabolism by post-transcriptional regulation of gene expression. However, there were few reports about bacterial sRNA as a biomarker of infection. To test the potential role of sRNA in indicating infection of Mycobacterium tuberculosis, total RNA were extracted from the filtrated bacterial cultural supernatant. After synthesis of cDNA by reverse transcription, four Mycobacterial sRNAs including ASdes, ASpks, AS1726, and AS1890, which have been experimentally confirmed by Kristine B in the year of 2009, were detected by real time PCR. The specificity was verified by sequencing of the amplified products. Moreover, we demonstrate that the presence of sRNA Asdes in plasma of 55.56% (15/27) TB patients and 25.00% (6/24) normal controls with BCG vaccination (P |
| doi_str_mv | 10.1016/j.bbrc.2018.04.165 |
| format | article |
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However, there were few reports about bacterial sRNA as a biomarker of infection. To test the potential role of sRNA in indicating infection of Mycobacterium tuberculosis, total RNA were extracted from the filtrated bacterial cultural supernatant. After synthesis of cDNA by reverse transcription, four Mycobacterial sRNAs including ASdes, ASpks, AS1726, and AS1890, which have been experimentally confirmed by Kristine B in the year of 2009, were detected by real time PCR. The specificity was verified by sequencing of the amplified products. Moreover, we demonstrate that the presence of sRNA Asdes in plasma of 55.56% (15/27) TB patients and 25.00% (6/24) normal controls with BCG vaccination (P < 0.05). Our results suggest that bacterial non-coding sRNA can be detected from either bacterial culture supernatants or patient's plasma. Detecting of Mycobacterial sRNA provides a rapid and relatively noninvasive approach for diagnosing disease and could be developed as a biomarker to identify patients with active tuberculosis to help make informed decisions about proper therapies.1</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2018.04.165</identifier><identifier>PMID: 29689271</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>60 APPLIED LIFE SCIENCES ; Animals ; Bacteriological Techniques ; Base Sequence ; BIOLOGICAL MARKERS ; Biomarker ; Cattle ; Humans ; Mycobacterium bovis - genetics ; Mycobacterium bovis - isolation & purification ; MYCOBACTERIUM TUBERCULOSIS ; Mycobacterium tuberculosis - genetics ; Mycobacterium tuberculosis - isolation & purification ; Real-Time Polymerase Chain Reaction ; RNA ; RNA, Bacterial - analysis ; RNA, Bacterial - blood ; RNA, Bacterial - genetics ; RNA, Small Untranslated - analysis ; RNA, Small Untranslated - blood ; RNA, Small Untranslated - genetics ; Sequence Alignment ; sRNA ; TB patients ; TUBERCULOSIS ; Tuberculosis - blood ; Tuberculosis - microbiology ; Tuberculosis, Bovine - blood ; Tuberculosis, Bovine - microbiology</subject><ispartof>Biochemical and biophysical research communications, 2018-09, Vol.503 (2), p.490-494</ispartof><rights>2018</rights><rights>Copyright © 2018. 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However, there were few reports about bacterial sRNA as a biomarker of infection. To test the potential role of sRNA in indicating infection of Mycobacterium tuberculosis, total RNA were extracted from the filtrated bacterial cultural supernatant. After synthesis of cDNA by reverse transcription, four Mycobacterial sRNAs including ASdes, ASpks, AS1726, and AS1890, which have been experimentally confirmed by Kristine B in the year of 2009, were detected by real time PCR. The specificity was verified by sequencing of the amplified products. Moreover, we demonstrate that the presence of sRNA Asdes in plasma of 55.56% (15/27) TB patients and 25.00% (6/24) normal controls with BCG vaccination (P < 0.05). Our results suggest that bacterial non-coding sRNA can be detected from either bacterial culture supernatants or patient's plasma. Detecting of Mycobacterial sRNA provides a rapid and relatively noninvasive approach for diagnosing disease and could be developed as a biomarker to identify patients with active tuberculosis to help make informed decisions about proper therapies.1</description><subject>60 APPLIED LIFE SCIENCES</subject><subject>Animals</subject><subject>Bacteriological Techniques</subject><subject>Base Sequence</subject><subject>BIOLOGICAL MARKERS</subject><subject>Biomarker</subject><subject>Cattle</subject><subject>Humans</subject><subject>Mycobacterium bovis - genetics</subject><subject>Mycobacterium bovis - isolation & purification</subject><subject>MYCOBACTERIUM TUBERCULOSIS</subject><subject>Mycobacterium tuberculosis - genetics</subject><subject>Mycobacterium tuberculosis - isolation & purification</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>RNA</subject><subject>RNA, Bacterial - analysis</subject><subject>RNA, Bacterial - blood</subject><subject>RNA, Bacterial - genetics</subject><subject>RNA, Small Untranslated - analysis</subject><subject>RNA, Small Untranslated - blood</subject><subject>RNA, Small Untranslated - genetics</subject><subject>Sequence Alignment</subject><subject>sRNA</subject><subject>TB patients</subject><subject>TUBERCULOSIS</subject><subject>Tuberculosis - blood</subject><subject>Tuberculosis - microbiology</subject><subject>Tuberculosis, Bovine - blood</subject><subject>Tuberculosis, Bovine - microbiology</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp9kU1rFTEUhoNY7G31D7iQgBs3M55kPgNuSm2rUCqIgruQZM5wc5lJxiRTuf_eDLfqzlUWed6Xc85DyGsGJQPWvj-UWgdTcmB9CXXJ2uYZ2TEQUHAG9XOyA4C24IL9OCcXMR4AGKtb8YKcc9H2gndsR44fMaFJ1jvqRzofjdfKJAxWTTTOapro14crah1Ne6T_vsw6pTUgjeuCwamkXKLKDXSZVE5tVYtKFl2K9JdNe5qD9hFpWjWGnPXRxpfkbFRTxFdP7yX5fnvz7fpTcf_l7vP11X1hqr5OxdiPDefjqLjulahGaPu6aQbgvaoH3SET3dAz3QyiE4yzukMwaug6Xeux4lxXl-TtqdfHZGU0Nu-7N965vLbkFYOm5VWm3p2oJfifK8YkZxsNTpNy6NcoOVQgWNU3G8pPqAk-xoCjXIKdVThKBnITIw9yEyM3MRJqmcXk0Jun_lXPOPyN_DGRgQ8nAPMtHi2GbVR0BgcbtkkHb__X_xsdyKBM</recordid><startdate>20180905</startdate><enddate>20180905</enddate><creator>Fu, Yingmei</creator><creator>Li, Wenjing</creator><creator>Wu, Zheng</creator><creator>Tao, Yuanmeihui</creator><creator>Wang, Xinyang</creator><creator>Wei, Jing</creator><creator>Jiang, Peidong</creator><creator>Wu, Jinyin</creator><creator>Zhang, Zhiwei</creator><creator>Zhang, Wenli</creator><creator>Zhao, Jizi</creator><creator>Zhang, Fengmin</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>20180905</creationdate><title>Detection of mycobacterial small RNA in the bacterial culture supernatant and plasma of patients with active tuberculosis</title><author>Fu, Yingmei ; Li, Wenjing ; Wu, Zheng ; Tao, Yuanmeihui ; Wang, Xinyang ; Wei, Jing ; Jiang, Peidong ; Wu, Jinyin ; Zhang, Zhiwei ; Zhang, Wenli ; Zhao, Jizi ; Zhang, Fengmin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-f8f522ffa2b8a93f068455d028a4db7e197d81b5d97912147e0cad77b4bf322b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>60 APPLIED LIFE SCIENCES</topic><topic>Animals</topic><topic>Bacteriological Techniques</topic><topic>Base Sequence</topic><topic>BIOLOGICAL MARKERS</topic><topic>Biomarker</topic><topic>Cattle</topic><topic>Humans</topic><topic>Mycobacterium bovis - genetics</topic><topic>Mycobacterium bovis - isolation & purification</topic><topic>MYCOBACTERIUM TUBERCULOSIS</topic><topic>Mycobacterium tuberculosis - genetics</topic><topic>Mycobacterium tuberculosis - isolation & purification</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>RNA</topic><topic>RNA, Bacterial - analysis</topic><topic>RNA, Bacterial - blood</topic><topic>RNA, Bacterial - genetics</topic><topic>RNA, Small Untranslated - analysis</topic><topic>RNA, Small Untranslated - blood</topic><topic>RNA, Small Untranslated - genetics</topic><topic>Sequence Alignment</topic><topic>sRNA</topic><topic>TB patients</topic><topic>TUBERCULOSIS</topic><topic>Tuberculosis - blood</topic><topic>Tuberculosis - microbiology</topic><topic>Tuberculosis, Bovine - blood</topic><topic>Tuberculosis, Bovine - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fu, Yingmei</creatorcontrib><creatorcontrib>Li, Wenjing</creatorcontrib><creatorcontrib>Wu, Zheng</creatorcontrib><creatorcontrib>Tao, Yuanmeihui</creatorcontrib><creatorcontrib>Wang, Xinyang</creatorcontrib><creatorcontrib>Wei, Jing</creatorcontrib><creatorcontrib>Jiang, Peidong</creatorcontrib><creatorcontrib>Wu, Jinyin</creatorcontrib><creatorcontrib>Zhang, Zhiwei</creatorcontrib><creatorcontrib>Zhang, Wenli</creatorcontrib><creatorcontrib>Zhao, Jizi</creatorcontrib><creatorcontrib>Zhang, Fengmin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fu, Yingmei</au><au>Li, Wenjing</au><au>Wu, Zheng</au><au>Tao, Yuanmeihui</au><au>Wang, Xinyang</au><au>Wei, Jing</au><au>Jiang, Peidong</au><au>Wu, Jinyin</au><au>Zhang, Zhiwei</au><au>Zhang, Wenli</au><au>Zhao, Jizi</au><au>Zhang, Fengmin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of mycobacterial small RNA in the bacterial culture supernatant and plasma of patients with active tuberculosis</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2018-09-05</date><risdate>2018</risdate><volume>503</volume><issue>2</issue><spage>490</spage><epage>494</epage><pages>490-494</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Bacterial small RNA (sRNA) has been shown to play an important role in control of bacteria virulence, stress response and physiological metabolism by post-transcriptional regulation of gene expression. However, there were few reports about bacterial sRNA as a biomarker of infection. To test the potential role of sRNA in indicating infection of Mycobacterium tuberculosis, total RNA were extracted from the filtrated bacterial cultural supernatant. After synthesis of cDNA by reverse transcription, four Mycobacterial sRNAs including ASdes, ASpks, AS1726, and AS1890, which have been experimentally confirmed by Kristine B in the year of 2009, were detected by real time PCR. The specificity was verified by sequencing of the amplified products. Moreover, we demonstrate that the presence of sRNA Asdes in plasma of 55.56% (15/27) TB patients and 25.00% (6/24) normal controls with BCG vaccination (P < 0.05). Our results suggest that bacterial non-coding sRNA can be detected from either bacterial culture supernatants or patient's plasma. Detecting of Mycobacterial sRNA provides a rapid and relatively noninvasive approach for diagnosing disease and could be developed as a biomarker to identify patients with active tuberculosis to help make informed decisions about proper therapies.1</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>29689271</pmid><doi>10.1016/j.bbrc.2018.04.165</doi><tpages>5</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 2018-09, Vol.503 (2), p.490-494 |
| issn | 0006-291X 1090-2104 |
| language | eng |
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| source | Elsevier |
| subjects | 60 APPLIED LIFE SCIENCES Animals Bacteriological Techniques Base Sequence BIOLOGICAL MARKERS Biomarker Cattle Humans Mycobacterium bovis - genetics Mycobacterium bovis - isolation & purification MYCOBACTERIUM TUBERCULOSIS Mycobacterium tuberculosis - genetics Mycobacterium tuberculosis - isolation & purification Real-Time Polymerase Chain Reaction RNA RNA, Bacterial - analysis RNA, Bacterial - blood RNA, Bacterial - genetics RNA, Small Untranslated - analysis RNA, Small Untranslated - blood RNA, Small Untranslated - genetics Sequence Alignment sRNA TB patients TUBERCULOSIS Tuberculosis - blood Tuberculosis - microbiology Tuberculosis, Bovine - blood Tuberculosis, Bovine - microbiology |
| title | Detection of mycobacterial small RNA in the bacterial culture supernatant and plasma of patients with active tuberculosis |
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