Chemoattractant receptors activate, recruit and capture G proteins for wide range chemotaxis

• Published in: Biochemical and biophysical research communications 2018-12, Vol.507 (1-4), p.304-310
• Main Authors: Miyanaga, Yukihiro, Kamimura, Yoichiro, Kuwayama, Hidekazu, Devreotes, Peter N., Ueda, Masahiro
• Format: Article
• Language: English
• Subjects: 60 APPLIED LIFE SCIENCES; AMP; Chemotaxis; Cyclic AMP - metabolism; Dictyostelium - cytology; Dictyostelium - metabolism; Dynamic range extension; Eukaryotic chemotaxis; G protein-coupled receptor; Gradient sensing; GTP-ASES; GTP-Binding Proteins - metabolism; Intracellular Space - metabolism; Protozoan Proteins - metabolism; RECEPTORS; Receptors, G-Protein-Coupled - metabolism; Signal Transduction; Single-molecule analysis
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2018.11.029

The wide range sensing of extracellular signals is a common feature of various sensory cells. Eukaryotic chemotactic cells driven by GPCRs and their cognate G proteins are one example. This system endows the cells directional motility towards their destination over long distances. There are several mechanisms to achieve the long dynamic range, including negative regulation of the receptors upon ligand interaction and spatial regulation of G proteins, as we found recently. However, these mechanisms are insufficient to explain the 105-fold range of chemotaxis seen in Dictyostelium. Here, we reveal that the receptor-mediated activation, recruitment, and capturing of G proteins mediate chemotactic signaling at the lower, middle and higher concentration ranges, respectively. These multiple mechanisms of G protein dynamics can successfully cover distinct ranges of ligand concentrations, resulting in seamless and broad chemotaxis. Furthermore, single-molecule imaging analysis showed that the activated Gα subunit forms an unconventional complex with the agonist-bound receptor. This complex formation of GPCR-Gα increased the membrane-binding time of individual Gα molecules and therefore resulted in the local accumulation of Gα. Our findings provide an additional chemotactic dynamic range mechanism in which multiple G protein dynamics positively contribute to the production of gradient information. [Display omitted] •Chemotactic GPCR forms an unconventional complex with the activated Gα.•Receptors activate, recruit and capture G proteins at different cAMP concentrations.•Capture of Gα can contribute to gradient sensing at high cAMP concentrations.