Natural Abundance Isotope Ratio Measurements of Organic Molecules Using 21 T FTICR MS

Subtle variations in stable isotope ratios at natural abundance are challenging to measure but can yield critical insights into biological, physical, and geochemical processes. Well-established methods, particularly multicollector, gas-source, or plasma isotope ratio mass spectrometry, are the gold...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2023-11, Vol.95 (47), p.17203-17211
Main Authors: Kew, William, Boiteau, Rene M., Eiler, John M., Paša-Tolić, Ljiljana, Moran, James J.
Format: Article
Language:English
Subjects:
07 ISOTOPE AND RADIATION SOURCES
21T-FTICR-MS
Abundance
Biological activity
Caffeine
Carbon 13
Cyclotron resonance
Fourier transforms
isotope ratio mass spectrometry
Isotope ratios
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Organic chemistry
Scientific imaging
Stable isotopes
Standard error
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Summary:Subtle variations in stable isotope ratios at natural abundance are challenging to measure but can yield critical insights into biological, physical, and geochemical processes. Well-established methods, particularly multicollector, gas-source, or plasma isotope ratio mass spectrometry, are the gold standard for stable isotope measurement, but inherent limitations in these approaches make them ill-suited to determining site-specific and multiply substituted isotopic abundances of all but a few compounds or to characterizing larger intact molecules. Fourier transform mass spectrometry, namely, Orbitrap mass spectrometry, has recently demonstrated the ability to measure natural abundance isotope ratios with chemically informative accuracy and precision. Here, we report the first use of Fourier transform ion cyclotron resonance mass spectrometry for the accurate (
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.3c01816