Incorporation of cytochrome oxidase into cardiolipin bilayers and induction of nonlamellar phases

Cytochrome oxidase from beef heart has been lipid-substituted with beef heart cardiolipin. The lipid phase behavior and protein aggregation state of the reconstituted complexes have been studied with 31P NMR, freeze-fracture electron microscopy, and saturation-transfer ESR of the spin-labeled protei...

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Bibliographic Details
Published in:Biochemistry (Easton) 1990-05, Vol.29 (21), p.5127-5132
Main Authors: Powell, Gary L, Knowles, Peter F, Marsh, Derek
Format: Article
Language:English
Subjects:
550601 - Medicine- Unsealed Radionuclides in Diagnostics
Analytical, structural and metabolic biochemistry
ANIMALS
Biological and medical sciences
BODY
CARDIOLIPIN
Cardiolipins - metabolism
CARDIOVASCULAR SYSTEM
CATTLE
CELL CONSTITUENTS
CELL MEMBRANES
CHROMATOGRAPHY
CYTOCHROME OXIDASE
cytochrome-c oxidase
Diffusion
DOMESTIC ANIMALS
ELECTRON MICROSCOPY
ELECTRON SPIN RESONANCE
Electron Transport Complex IV - metabolism
Enzyme Induction
ENZYMES
Enzymes and enzyme inhibitors
ESTERS
Freeze Fracturing
Fundamental and applied biological sciences. Psychology
HAEM DEHYDROGENASES
HEART
ISOTOPES
LIGHT NUCLEI
Lipid Bilayers - metabolism
LIPIDS
MAGNETIC RESONANCE
Magnetic Resonance Spectroscopy
MAMMALS
MEMBRANES
MICROSCOPY
Myocardium - enzymology
Myocardium - ultrastructure
NUCLEAR MAGNETIC RESONANCE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
OXIDOREDUCTASES
PHASE STUDIES
PHOSPHOLIPIDS
PHOSPHORUS 31
PHOSPHORUS ISOTOPES
Protein Conformation
RADIOLOGY AND NUCLEAR MEDICINE
RESONANCE
RUMINANTS
SEPARATION PROCESSES
STABLE ISOTOPES
Temperature
THIN-LAYER CHROMATOGRAPHY
VERTEBRATES
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Summary:Cytochrome oxidase from beef heart has been lipid-substituted with beef heart cardiolipin. The lipid phase behavior and protein aggregation state of the reconstituted complexes have been studied with 31P NMR, freeze-fracture electron microscopy, and saturation-transfer ESR of the spin-labeled protein. In the absence of salt, the lipid has a lamellar arrangement, and the protein is integrated and uniformly distributed in the membrane vesicles and undergoes rapid rotational diffusion. The presence of the protein stabilizes the cardiolipin lamellar phase against salt-induced transitions to the inverted hexagonal phase. The threshold salt concentration becomes higher and the extent of conversion becomes lower with decreasing lipid:protein ratio. In high salt, lamellar-phase lipid with integrated protein coexists with hexagonal-phase lipid free of protein, and the rotational diffusion of the protein is drastically reduced as a result of the high packing density.
ISSN:0006-2960
1520-4995
DOI:10.1021/bi00473a018